The present study is a trial to improve the reproductive performance of Egyptian Buffaloes following the administration of probiotics during late gestation and early postpartum period. Fifteen lactating buffaloes at one-month pre-partum were divided randomly into three groups (5 each) until five months postpartum. The control group fed basal diet without supplements (G1), the 2nd group (G2) was given the basal diet plus 20 g\h\d of Saccharomyces cerevisiae yeast culture (YC), the 3rd group (G3) was given the basal diet plus 20 g\h\d of Lactobacillus acidophilus (LB). Blood samples were collected monthly during the postpartum period from calving into the 5th month for estimation of enzymes activities, hematological and blood parameters as well as reproductive measurements, while colostrum samples were taken from dame on days 1, 2 and 3 postpartum to measure the concentration of immunoglobulin. Results showed that the values of all hematological parameters were significantly improve in G3 followed by G2 than control. Also, Data indicated that the values of all biochemical blood parameters for buffaloes treated with LB in G3 were significantly higher than G2, while G1 was lower significantly than others treated groups. Enzymes activities and reproductive measurements percentage increased insignificantly in G3 and G2 compared to G1. There were highly significant IgA,IgG,IgM concentrations in colostrum of dame in G3 followed by G2 than control. It could be concluded that LB supplementation to rations of buffaloes had beneficial effects on health status, immunity response, and the reproductive performance under the local farm feeding condition in Egypt.