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177577

Genetic variations in some Egyptian Zea mexicana genotypes based on RAPD and AFLP markers

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Last updated: 25 Dec 2024

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Abstract

Maize-Teosinte hybrids are of significant interest to maize breeders as a resource of genetic diversity during the maize domestication process as a genetic pool for maize improvement. This study examines the genetic diversity and population structure of 16 Zea mexicana populations which are currently active in Zea breeding programs in Egypt using Random Amplified Polymorphic DNA (RAPD) and Amplified Fragment Length Polymorphism (AFLP) markers. RAPD analysis (14 primers) produced 141 bands; out of which, 102 (72.3%) were polymorphic. On the other hand, AFLP (5 primer combinations) yielded 276 peaks including 267 (96.7%) polymorphic ones with an average of 53.4 peaks per primer combination. A total of 11 unique RAPD markers were created by 6 primers and identified 8 genotypes. The five primer combinations generated 56 unique amplicons that successfully distinguished 12 out of the 16 genotypes tested. Furthermore, the number of observed alleles (Na), effective multiplex ratio (EMR), and polymorphic information content (PIC) indices showed higher values for AFLP (2.00, 53.4, and 0.21) than for RAPD (1.67, 7.3, and 0.15). Cluster analysis based on Nei and Li genetic distance and an Unweighted Pair Group Method with Arithmetic Mean (UPGMA) revealed 5 main clusters representing the 16 Zea mexicana confirming the population structure analysis obtained. High variability of the studied teosinte genotypes using RAPD and AFLP markers will provide valuable tools for Zea mexicana breeding programs in Egypt.

DOI

10.21608/jbaar.2021.177577

Keywords

Zea mexicana, AFLP, RAPD, molecular markers, Genetic diversity

Authors

First Name

K.

Last Name

Radwan

MiddleName

H.

Affiliation

Agricultural Genetic Engineering Research Institute (AGERI), Agricultural Research Center (ARC), Giza -12619, Egypt/National biotechnology Network of Expertise, ASRT, Egypt

Email

khradwan@gmail.com

City

-

Orcid

-

First Name

G.

Last Name

Abdelfattah

MiddleName

A.

Affiliation

Agricultural Genetic Engineering Research Institute (AGERI), Agricultural Research Center (ARC), Giza -12619, Egypt/Department of Pharmacology and Toxicology, College of Pharmacy, Prince Sattam Bin Abdulaziz University, Al-Kharj, KSA

Email

-

City

-

Orcid

-

First Name

M.

Last Name

Badawi

MiddleName

A.

Affiliation

Agricultural Genetic Engineering Research Institute (AGERI), Agricultural Research Center (ARC), Giza -12619, Egypt/Dép. des Sciences Biologiques, Univ. du Québec à Montréal, Montréal, QC H3C 3P8, Canada

Email

-

City

-

Orcid

-

First Name

E.

Last Name

Zayed

MiddleName

M.

Affiliation

Cell Study Research Department (CSRD), Field Crops Research Institute, Agriculture Research Center (ARC), Giza 12619, Egypt

Email

-

City

-

Orcid

-

First Name

M.

Last Name

Tarrd

MiddleName

M.

Affiliation

Forage Crops Research Department (FCRD), Field Crops Research Institute, Agriculture Research Center (ARC), Giza-12619, Egypt

Email

-

City

-

Orcid

-

First Name

Manal

Last Name

El-Baghdady

MiddleName

M. S.

Affiliation

Agricultural Genetic Engineering Research Institute (AGERI), Agricultural Research Center (ARC), Giza -12619, Egypt

Email

-

City

-

Orcid

-

First Name

Reem

Last Name

Abd El-Maksoud

MiddleName

M.

Affiliation

Agricultural Genetic Engineering Research Institute (AGERI), Agricultural Research Center (ARC), Giza -12619, Egypt

Email

-

City

-

Orcid

-

Volume

7

Article Issue

2

Related Issue

25802

Issue Date

2021-06-01

Receive Date

2021-04-06

Publish Date

2021-06-01

Page Start

77

Page End

92

Print ISSN

2356-9174

Online ISSN

2356-9182

Article File

JBAAR_Volume 7_Issue 2_Pages 77-92.pdf

PDF . 914.0kB

Link

https://jbaar.journals.ekb.eg/article_177577.html

Detail API

https://jbaar.journals.ekb.eg/service?article_code=177577

Order

5

Type

Original Article

Type Code

1,272

Publication Type

Journal

Publication Title

Journal of Bioscience and Applied Research

Publication Link

https://jbaar.journals.ekb.eg/

MainTitle

Genetic variations in some Egyptian Zea mexicana genotypes based on RAPD and AFLP markers

Details

Type

Article

Created At

22 Jan 2023