Grape seeds are waste products of the winery and grape juice and are good sources of phytochemicals. The present study aimed to investigate the qualitative and quantitative characterization of Egyptian grape seeds by microwave-assisted extraction (MAE), which was determined by high-performance liquid chromatography coupled to electrospray quadrupole-time of flight mass spectrometry (HPLC–ESI-QTOF-MS). According to the data compiled, grape (Vitis vinifera) seeds sample presented the highest levels in flavonoids and other compounds such as Carnosol, rosmaridiphenol, rosmadial, rosmarinic acid, and carnosic acid. On the other hand, higher contents in triterpenes were found in the extracts of rosemary. The current results indicate that the ethanolic extract from Egyptian grape seeds possessed radical-scavenging and antioxidant activities where about 17.442 mg GAE/g of total phenols, 6.687 mg CE/g of total flavonoids and 81.506 mg TE/g DPPH as antioxidants activity in grape seeds. grape (Vitis vinifera) seeds are very rich sources of flavonoids and typical compounds of grape seeds, such as Catachine, Gallic acid, Protochatchuic acid and Syrngic acid which are compounds with many biological properties, especially antioxidant. On the other hand, the Qurecetin were highly represented that indicate the anticancer and anti-inflammatory properties of grape seeds. This aspect should be studied in depth in future research.