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141374

Combining nuclear matrix protein-52, collagen III, and matrix metalloproteinase-1 for more effective breast cancer early detection

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Last updated: 25 Dec 2024

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Abstract

Aim: Improvement of breast cancer (BrCa) control will be markedly supported by early detection. Owing to limitations of current diagnostic tools like mammography and ultrasound and lack of existing confirmed BrCa biomarkers, this study concerned the evaluation of some potential biomarkers and their combination in BrCa detection. Methods: Three hundred participant women; 200 with BrCa patients, 50 with benign breast diseases, and 50 healthy individuals were enrolled in this study. Serum levels of nuclear matrix protein-52 (NMP-52), collagen III, and matrix metalloproteinase-1 (MMP-1) were determined by ELISA. Results: Mean levels of NMP-52 (9.83±1.1 μg/ml), collagen III (22.6±3.2 μg/mL) and MMP-1 (3.6±0.3 μg/mL) in BrCa patients were significantly higher (P<0.0001) than benign (5.8±0.7, 12.2±1.3 and 2.6±0.23 μg/mL, respectively) and healthy (1.2±0.1, 6.0±0.2 and 1.66±0.04 μg/mL, respectively) groups. Also, these levels were associated with the tumor progression and may reflect the BrCa disease severity, high serum levels of these markers have been associated with tumor advanced stages (T3-T4), high grade (G3), and large size (>2cm). Diagnostic scores combined these markers revealed valuable power (AUC=0.83, 78% sensitivity, 75% specificity) in BrCa diagnosis. This power is not markedly influenced in the detection of early tumor stages (Tis-T2), low grade (G1-G2), lesser tumor size ≤2 cm, and negative lymph nodes status (AUC=0.79, 0.74, 0.74, and 0.85, respectively). Conclusions: Combined use of NMP-52, collagen III, and MMP-1 can serve as a potential biomarker for BrCa diagnosis. This combination is likely to improve the clinical early tumor diagnosis.

DOI

10.21608/jbaar.2019.141374

Keywords

breast cancer, collagen, matrix metalloproteinase, Nuclear matrix protein, Score

Authors

First Name

Abdelfattah

Last Name

Attallah

MiddleName

M.

Affiliation

Research & Development Department, Biotechnology Research Center, New Damietta, Egypt

Email

amattallah@hotmail.com

City

-

Orcid

-

First Name

Mohamed

Last Name

El-Far

MiddleName

-

Affiliation

Chemistry Department, Faculty of Science, Mansoura University, Mansoura, Egypt

Email

-

City

-

Orcid

-

First Name

Mohy

Last Name

Abdel Fatah

MiddleName

E.

Affiliation

Chemistry Department, Faculty of Science, Suez Canal University, Ismailia, Egypt

Email

-

City

-

Orcid

-

First Name

Mohamed

Last Name

Omran

MiddleName

M.

Affiliation

Chemistry Department, Faculty of Science, Helwan University, Cairo, Egypt

Email

-

City

-

Orcid

-

First Name

Mohamed

Last Name

Abdelrazek

MiddleName

A.

Affiliation

Research & Development Department, Biotechnology Research Center, New Damietta, Egypt

Email

maabdelrazek@yahoo.com

City

-

Orcid

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First Name

Gamal.

Last Name

Abdelhameed

MiddleName

E

Affiliation

Research & Development Department, Biotechnology Research Center, New Damietta, Egypt

Email

-

City

-

Orcid

-

First Name

Kareem

Last Name

Attallah

MiddleName

A.

Affiliation

Research & Development Department, Biotechnology Research Center, New Damietta, Egypt

Email

-

City

-

Orcid

-

First Name

Nada

Last Name

Ahmed

MiddleName

A.

Affiliation

Research & Development Department, Biotechnology Research Center, New Damietta, Egypt

Email

-

City

-

Orcid

-

First Name

Esraa

Last Name

El-Sayes

MiddleName

A.

Affiliation

Research & Development Department, Biotechnology Research Center, New Damietta, Egypt

Email

-

City

-

Orcid

-

First Name

Fatma

Last Name

Khedr

MiddleName

M.

Affiliation

Research & Development Department, Biotechnology Research Center, New Damietta, Egypt

Email

-

City

-

Orcid

-

First Name

Ibrahim

Last Name

El-Dosoky

MiddleName

-

Affiliation

Pathology Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt

Email

-

City

-

Orcid

-

Volume

5

Article Issue

2

Related Issue

20540

Issue Date

2019-06-01

Receive Date

2019-03-03

Publish Date

2019-05-19

Page Start

212

Page End

221

Print ISSN

2356-9174

Online ISSN

2356-9182

Article File

JBAAR_Volume 5_Issue 2_Pages 212-221.pdf

PDF . 593.7kB

Link

https://jbaar.journals.ekb.eg/article_141374.html

Detail API

https://jbaar.journals.ekb.eg/service?article_code=141374

Order

7

Type

Original Article

Type Code

1,272

Publication Type

Journal

Publication Title

Journal of Bioscience and Applied Research

Publication Link

https://jbaar.journals.ekb.eg/

MainTitle

Combining nuclear matrix protein-52, collagen III, and matrix metalloproteinase-1 for more effective breast cancer early detection

Details

Type

Article

Created At

22 Jan 2023