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109489

Effect of anti-sca-1+ on diabetic rat histological, immunohistochemical and biochemical evaluation.

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Last updated: 25 Dec 2024

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Abstract

Diabetes mellitus is a disease of metabolic dysregulation most notable glucose metabolism accompanied by long term complications. The present study was carried out to investigate the potential effect of Anti-Sca-1+ stem cells on type 1 diabetes induced in albino rats. The study was carried on 7 male and 24 female Albino rats (150 ± 5g). The female albino rats were divided into three main groups: normal control, diabetic group and diabetic rats treated by Anti-Sca-1+. Induction of diabetic rats was carried out by single dose injection of fasting rats for 48 h with a diluted solution of 40 mg/kg of alloxan monohydrate (2%) in freshly prepared saline solution. Diabetic rats treated group was slaughtered into two phases; the first phase after 15 days of treatment, and the second phase after 30 days of treatment. Tissue samples were separated and kept in absolute 10% formalin solution for histological and immunohistochemical studies. SRY gene expression and biochemical study of glucose tolerance, c-peptide, insulin, glycated hemoglobin and glucose transporter 2 was evaluated. The results showed that, treatment of diabetic rats, with derivative bone marrow (Anti-Sca-1+) could greatly ameliorate glucose, insulin, c-peptide, HbA1c and glut2, Hafter 15 and 30 days of treatment, as compared to normal control group and these were confirmed by histological and immunological investigations, which indicate significant progress in the pancreas tissue, by marked significant increase of pancreatic islets size and improvement in the acinar cells after 30 days of treatment by Anti-Sca-1+.

DOI

10.21608/jbaar.2016.109489

Keywords

Diabetes mellitus, Mesenchymal cells, AntiSca-1+, pancreas, Insulin, DNA

Authors

First Name

Bahgat

Last Name

El-Fiky

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Affiliation

Department of Animal Biotechnology, Genetic Engineering and Biotechnology Institute University of Sadat City

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First Name

Saber A.

Last Name

Sakr

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Affiliation

Department of Zoology, Faculty of Science, Menoufia University, Shebin El-kom, Egypt.

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First Name

Sobhy Hassab

Last Name

El Nabi

MiddleName

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Affiliation

Department of Zoology, Faculty of Science, Menoufia University, Shebin El-kom, Egypt.

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First Name

Mona

Last Name

El Gawish

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Affiliation

Departments of Biochemistry, National Center for Radiation Research Atomic Energy Authority, Egypt.

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First Name

Asmaa

Last Name

Uonis

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Affiliation

Departments of Biochemistry, National Center for Radiation Research Atomic Energy Authority, Egypt.

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Orcid

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First Name

Ahmed

Last Name

EL Kattan

MiddleName

-

Affiliation

Departments of Biochemistry, National Center for Radiation Research Atomic Energy Authority, Egypt.

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Volume

2

Article Issue

10

Related Issue

16640

Issue Date

2016-10-01

Receive Date

2016-09-07

Publish Date

2016-10-20

Page Start

641

Page End

650

Print ISSN

2356-9174

Online ISSN

2356-9182

Article File

JBAAR_Volume 2_Issue 10_Pages 641-650.pdf

PDF . 989.0kB

Link

https://jbaar.journals.ekb.eg/article_109489.html

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https://jbaar.journals.ekb.eg/service?article_code=109489

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Original Article

Type Code

1,272

Publication Type

Journal

Publication Title

Journal of Bioscience and Applied Research

Publication Link

https://jbaar.journals.ekb.eg/

MainTitle

Effect of anti-sca-1+ on diabetic rat histological, immunohistochemical and biochemical evaluation.

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Article

Created At

22 Jan 2023