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Pomegranate peel Extract Protects Cadmium-induced nephrotoxicity in albino mice.

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Last updated: 25 Dec 2024

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Abstract

Cadmium chloride (CdCl2) is a toxicant heavy metal displays adverse properties in humans creating public health risks. Pomegranate (Punica granatum L.) is widely known as antimicrobial and antioxidant. This study investigated the cadmium-induced structural effects in mice and evaluated the beneficial effect of alcoholic extract of P. granatum fruit peel (PPE) to protective CdCl2 nephrotoxicity. Animals were divided into 4 groups; group 1: control, group 2: given 25ml/kg PPE, group 3: given CdCl2 at a dose level of 2mg/kg, and Group 4: given CdCl2 and PPE. The animals were given the previous treatment daily for 14days. CdCl2 intoxication led to obvious many histopathological alterations in kidney glomeruli accompanied with wide and congested blood vessels, renal tubules missed their distinct form with cytoplasmic vacuolation of their epithelial cells and pyknotic nuclei and leucocytes cells infiltration in the intertubular spaces. On the other hand, the immunohistochemical staining of antiapoptotic Bcl-2 and α-smooth muscle actin (α-SMA) expressions were positive after CdCl2 exposure compared with the control group. Ultrastructure observations revealed thickening of the glomerular basement membrane and fusion of the podocytes foot processes, tubular epithelial cells vacoulation with pyknotic nuclei, perforation and vacoulation of mitochondria, deterioration of endoplasmic reticulum, and increase of lysosomes. CdCl2-exposure accompanied by increased level of serum urea, creatinine and blood urea nitrogen (BUN) as well as significant increase in malondialdehyde (MDA) besides decreased of the total antioxidant capacity (TAC) level. In contrast, co-administration of PPE plus CdCl2 ameliorated these parameters around the normal levels. It contributed the improvement by the histological, ultrastructure, and decreased Bcl-2 and α smooth muscle protein expression, and kidney function through a significant decrease in urea, creatinine, and BUN, reduced the level of serum MDA as lipid peroxidation marker and restored the altered antioxidant system activity. It was concluded that Cd-induced nephrotoxicity at a dose level 2 mg/kg b.w. in mice. The PPE may be involved in the protection of toxicity displayed by CdCl2 induction attributed to the high antioxidant capacity.

DOI

10.21608/jbaar.2016.108529

Keywords

Cadmium chloride, Nephrotoxicity, pomegranate, histopathology, Immunohistochemistry, Ultrastructure, antioxidant

Authors

First Name

Amal A.

Last Name

El-Daly

MiddleName

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Affiliation

Zoology Department, Faculty of Science, Benha University, Benha, Egypt

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Volume

2

Article Issue

6

Related Issue

16400

Issue Date

2016-06-01

Receive Date

2016-05-17

Publish Date

2016-06-22

Page Start

362

Page End

375

Print ISSN

2356-9174

Online ISSN

2356-9182

Link

https://jbaar.journals.ekb.eg/article_108529.html

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https://jbaar.journals.ekb.eg/service?article_code=108529

Order

3

Type

Original Article

Type Code

1,272

Publication Type

Journal

Publication Title

Journal of Bioscience and Applied Research

Publication Link

https://jbaar.journals.ekb.eg/

MainTitle

Pomegranate peel Extract Protects Cadmium-induced nephrotoxicity in albino mice.

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Article

Created At

22 Jan 2023