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107538

Development of cytochrome-c-oxidase 1 specific primers for genetic discrimination of the European eel Anguilla anguilla (Linnaeus, 1758).

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Last updated: 04 Jan 2025

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Abstract

The European eel Anguilla anguilla (Anguillidae, Anguilliformes) is amongst the most peculiar, economically-important fish species in the Egyptian and Mediterranean waters. Very rare studies are available about its populations in the Mediterranean. In order to investigate this issue, we designed cytochrome-c-oxidase subunit 1 (COI) gene-specific primers for A. anguilla. These primers targeted the amplification of a 750 bp fragment towards the 5` extremity of COI. The primers were tested for A. anguilla elvers collected from Rachid Estuary in Egypt. They succeeded to amplify the targeted fragment. Using Forensically Informative Nucleotide Sequences (FINS) analysis and BLAST comparisons, the sequences were successfully assigned to A. anguilla, with clear segregation from other Anguilla species. The primers designed and used in this study can be then suggested as successful alternatives for universal primers commonly used in European eel barcoding.

DOI

10.21608/jbaar.2016.107538

Keywords

Anguilla Anguilla, CO1, fins, molecular markers, population genetics, primers

Authors

First Name

Khaled Mohammed-

Last Name

Geba

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Affiliation

Molecular Biology and Genetic Engineering Division, Department of Zoology, Faculty of Sciences, Menoufia University, Shebin El- Kom, Menoufia, Egypt.

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First Name

Sobhy El-Sayed

Last Name

Hassab El-Nabi

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Affiliation

Molecular Biology and Genetic Engineering Division, Department of Zoology, Faculty of Sciences, Menoufia University, Shebin El- Kom, Menoufia, Egypt.

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First Name

Marwa Said

Last Name

El-Desoky

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-

Affiliation

Molecular Biology and Genetic Engineering Division, Department of Zoology, Faculty of Sciences, Menoufia University, Shebin El- Kom, Menoufia, Egypt.

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Volume

2

Article Issue

4

Related Issue

16281

Issue Date

2016-04-01

Receive Date

2016-03-16

Publish Date

2016-04-23

Page Start

258

Page End

262

Print ISSN

2356-9174

Online ISSN

2356-9182

Article File

JBAAR_Volume 2_Issue 4_Pages 258-262.pdf

PDF . 1.1MB

Link

https://jbaar.journals.ekb.eg/article_107538.html

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https://jbaar.journals.ekb.eg/service?article_code=107538

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4

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Original Article

Type Code

1,272

Publication Type

Journal

Publication Title

Journal of Bioscience and Applied Research

Publication Link

https://jbaar.journals.ekb.eg/

MainTitle

Development of cytochrome-c-oxidase 1 specific primers for genetic discrimination of the European eel Anguilla anguilla (Linnaeus, 1758).

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Article

Created At

22 Jan 2023