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106909

Effect of L-arginine on methotrexate induced hepatotoxicity in albino rats.

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Last updated: 25 Dec 2024

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Abstract

Methotrexate (MTX) is commonly used in the treatment of many different types of cancer and inflammatory diseases. Its cytotoxic nature also lends a substantial risk of life-threatening side effects. L-arginine is beneficial in the treatment of hepatic injury, hepatic cirrhosis and fatty liver degeneration. The present work aims to study the effect of L-arginine on hepatotoxicity of methotrexate in albino rats. Five groups of albino rats were used. Group I: control. Group II: rats were administered (MTX) in a daily oral dose of 0.45 mg/kg, for 28 days. Group III: rats were administered L-arginine in a daily oral dose of 300 mg/kg, for 28 days. Group IV: rats were received L- arginine 2 hrs before (MTX). Group V: rats were received L-arginine 2 hrs after (MTX). The results revealed different histopathological changes in liver of MTX-treated rats such as focal areas of necrosis and increased numbers of activated Kupffer cells, an apparent increase in the amount of collagen fibers and strong immunoreactive expression of α- SMA. Biochemical results revealed a significant increase in the serum levels of ALT, AST, bilirubin and decreasing the level of antioxidant enzymes. L-agrinine minimized the hepatotoxicity of MTX by decreasing the level of ALT, AST and bilirubin, MDA and increasing the antioxidant enzymes. It is concluded that L-arginine protects liver from hepatotoxicity of methotrexate and this due to its antioxidant activity.

DOI

10.21608/jbaar.2016.106909

Keywords

Liver, Methotrexate, L-arginine, α- SMA, ALT, AST, Bilirubin, SOD, GSH and MDA

Authors

First Name

Ashour A - S

Last Name

Abdel-Mawla

MiddleName

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Affiliation

Histochemistry and Cell Biology Department, Medical Research Institute Alexandria University

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First Name

Safia M

Last Name

Hassan

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-

Affiliation

Histochemistry and Cell Biology Department, Medical Research Institute Alexandria University

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Orcid

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First Name

Ekram N

Last Name

Abd Al-Haleem

MiddleName

-

Affiliation

Pharmacology and Toxicology Department Faculty of Pharmacy (Girls), Al- Azhar University

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Orcid

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First Name

Safeyah Z

Last Name

ElHangoor

MiddleName

-

Affiliation

Pharmacology and Toxicology Department Faculty of Pharmacy (Girls), Al- Azhar University

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Volume

2

Article Issue

2

Related Issue

16119

Issue Date

2016-02-01

Receive Date

2016-01-15

Publish Date

2016-02-01

Page Start

88

Page End

99

Print ISSN

2356-9174

Online ISSN

2356-9182

Article File

JBAAR_Volume 2_Issue 2_Pages 88-99.pdf

PDF . 1.4MB

Link

https://jbaar.journals.ekb.eg/article_106909.html

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https://jbaar.journals.ekb.eg/service?article_code=106909

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Original Article

Type Code

1,272

Publication Type

Journal

Publication Title

Journal of Bioscience and Applied Research

Publication Link

https://jbaar.journals.ekb.eg/

MainTitle

Effect of L-arginine on methotrexate induced hepatotoxicity in albino rats.

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Article

Created At

22 Jan 2023