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105946

Antitumor activity of two Streptomyces extracts (Ag18 & Ag20) on Ehrlich ascites tumor in mice in vitro and in vivo studies

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Last updated: 25 Dec 2024

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Abstract

This study aimed to evaluate the potent antitumor activity of metabolites from Streptomyces as antitumor activity using in vitro and in vivo studies. The Streptomyces were purely isolated from the soil samples, processed to obtain their extracts. Twenty Streptomyces extracts were obtained. Two isolates of Streptomyces were characterized morphologically, physiologically, biochemically and 16sr RNA and they named Streptomyces strain Ag18 and Streptomyces strain Ag 20. female albino mice were used in the present study. The mice were divided into 4 groups (6 animals each was control, injected subcutaneously with an isotonic saline solution. The 2nd, injected subcutaneously with Ehrlich ascites tumor cells (EAC). Both the 3rd and 4 injected subcutaneously with Ehrlich ascites tumor cells (EAC) diluted with an isotonic saline solution (SS) tumor formation, (7-10) days after inoculation with EAC cells, animals were locally injected subcutaneous Streptomyces extract, Ag18 and Ag20.The results revealed that only two of the twenty Streptomyces and Ag20) showed their potent antitumor activity against Ehrlich ascites carcinoma cells in vitro. AG18 and AG20 Streptomyces extracts also modulated the mice body weight towards the normal values significantly(p< 0.001) and reduced the tumor volume with a very highly significant change (p< 0.001). The histological study confirmed the above mentioned result, where the kidney and liver of the mice injected with EAC returned to their normal structure after injection with Streptomyces extracts. It is concluded from this study that bioactive product ofStreptomycesAg18 & Ag20 have antitumor activity

DOI

10.21608/jbaar.2015.105946

Keywords

EAC (Ehrlich Ascites Carcinoma) cells, Histological studies, in vitro & in vivo studies mice, Streptomyces Ag18 & Ag20

Authors

First Name

Amany

Last Name

Gaber

MiddleName

-

Affiliation

Microbial Biotechnology Department, Genetic Engineering & Biotechnology Institute, Sadat city University, Sadat City, Egypt

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Orcid

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First Name

Osama

Last Name

Badr

MiddleName

M.

Affiliation

Animal Biotechnology Department, Genetic Engineering & Biotechnology Institute Sadat City University, Sadat city, Egypt

Email

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City

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Orcid

-

First Name

Saad

Last Name

Emara

MiddleName

A.

Affiliation

Cytology & Histology Department, Faculty of Veterinary Medicine, Sadat city University, Sadat city, Egypt

Email

-

City

-

Orcid

-

First Name

Atef

Last Name

Ibrahim

MiddleName

M.

Affiliation

Microbial Biotechnology Department, Genetic Engineering & Biotechnology Institute, Sadat city University, Sadat City, Egypt

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-

City

-

Orcid

-

Volume

1

Article Issue

1

Related Issue

16033

Issue Date

2015-03-01

Receive Date

2015-02-23

Publish Date

2015-03-01

Page Start

20

Page End

29

Print ISSN

2356-9174

Online ISSN

2356-9182

Article File

JBAAR_Volume 1_Issue 1_Pages 20-29.pdf

PDF . 8.4MB

Link

https://jbaar.journals.ekb.eg/article_105946.html

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https://jbaar.journals.ekb.eg/service?article_code=105946

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3

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Original Article

Type Code

1,272

Publication Type

Journal

Publication Title

Journal of Bioscience and Applied Research

Publication Link

https://jbaar.journals.ekb.eg/

MainTitle

Antitumor activity of two Streptomyces extracts (Ag18 & Ag20) on Ehrlich ascites tumor in mice in vitro and in vivo studies

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Type

Article

Created At

22 Jan 2023