The current demonstrated the cytototoxic effects of alparazolam on the vital organs of rats. Animal were divided into two groups ; G1 : normal control (not received any drug), G2 : alpazolam treated group. The effects of this drug toxicity on different tissue vital organs tissues (liver,kidney and heart ) were studied after 10,20 and30 days from drug administration. The results revealed that administration of alprazolam induces oxidative tissue damage indicated by significant increase in the activity of xanthine oxidase (XO) with concomitant elevated level of nitric oxide(NO) in liver kidney and heart of alprazolam-treated rats in comparison with normal animals. The increment of such oxidative tissue damage markers was accompanied by increased malondialdehyde (MDA, index of lipid peroxidation) in kidney, and decrease in adenosine triphosphatatase (ATPase) and lactate dehdrogenase(LDH)activities in cardiac tissue. The oxidative tissue damage induced in liver in response to alprazolam ingestion was supported by a depletion in the activity of hepatic sobitol dehydrogenase(SD)copled with elevation in serum enzymes, aspirate aminotransferase (AST), alanine .