In the present study 34 different animals at Alexandria Zoo were examined parasitologicaly for detection of Gastrointestinal parasites. The investigation of zoo animals( 3 Adult Common Zebra 12 Barbary sheep 4 Bactrian camels 1Nilgai ,5African Lions 2Chimpanzees 1Giraffe 1 Elephant, 4 Lama and 1 Gazelle) revealed that the overall prevalence of parasitic infection was 43%, Seven species of parasites. Were 4 helminthes from which Trichostrongyluscolumriformis Ostertagia circumcincta from lama at 35.3% & 33.2% respectively. Strongyloides papillosus ,from Barbary sheep 78% and Toxocara cati from African lions (23%) . And 3 protozoa Eimeria species from Bactrian camels (21.1%), Entamoeba histolytica cysts and Cryptosporidium oocysts from Chimpanzees at an incidence of 43.5% and 23.4% respectively.While the collected samples from common zebra, Nilgai, Gazalle, Giraffe and Elephant were free from any intestinal p < /p>