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153645

Isolation and identification of Marek’s disease virus (MDV) from feather follicle epithelium and internal organs of diseased chickens in Dakahlia Governorate, Egypt

Article

Last updated: 04 Jan 2025

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Tags

Virology

Abstract

In the present study, a total of 16 samples including feather follicle epithelium, ovary, spleen and kidney (4 samples for each organ) were collected from diseased chicken flocks suspected to be infected with Marek's disease virus (MDV) at Dakahlia Governorate, Egypt during the period from October 2016 to October 2017. Each sample was pooled randomly from three to five birds (90 to 360 days old). The isolation of the suspected virus from the collected samples was carried out via chorioallantoic membranes (CAMs) of 12 days old embryonated chicken eggs (ECEs). Three egg passages were carried out for each sample. Hyperimmune serum was prepared against standard MDV. MDV in both field and egg passaged samples (after 3rd passage) was identified by agar gel precipitation test (AGPT) and indirect fluorescence antibody test (IFAT). Molecular identification of virus was carried out by conventional polymerase chain reaction (PCR) and real- time PCR in four selected samples. The results revealed that 14 samples (87.5%) including 4 (100%) samples from feather follicle epithelium, ovary and kidney and 2 (50%) samples from spleen, showed positive results in virus isolation after 3rd passage. The positive results percentage by AGPT for field samples were 50% (8 out of 16 samples), while after the 3rd passage in ECEs were 37.5% (6 out of 16 samples) and the positive results percentage by IFAT for field samples were 62.5% (10 out of 16 samples), while after the 3rd passage in ECEs were 81.25 % (13 out of 16 samples). Viral nucleic acid was detected in all selected samples by conventional and real- time PCR. The results indicate that feather follicle epithelium is the best organ for MDV detection. IFAT is superior over AGPT in virus detection. Conventional and real - time PCR could be efficiently used for molecular detection of the virus.

DOI

10.21608/mvmj.2019.22.102

Keywords

Marek’s disease virus, virus isolation, agar gel precipitation test, indirect fluorescence antibody test, conventional and real-time (PCR)

Authors

First Name

Aly

Last Name

El-Kenawy

MiddleName

-

Affiliation

Department of Virology, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt

Email

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City

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Orcid

-

First Name

Emad

Last Name

A

MiddleName

-

Affiliation

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Email

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City

-

Orcid

-

First Name

Mohamed

Last Name

El-Tholoth

MiddleName

-

Affiliation

Department of Virology, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt

Email

-

City

-

Orcid

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Volume

20

Article Issue

2

Related Issue

22627

Issue Date

2019-06-01

Receive Date

2019-03-04

Publish Date

2019-06-25

Page Start

6

Page End

11

Print ISSN

1110-7219

Online ISSN

2682-2512

Article File

MVMJ_Volume 20_Issue 2_Pages 6-11.pdf

PDF . 917.0kB

Link

https://mvmj.journals.ekb.eg/article_153645.html

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https://mvmj.journals.ekb.eg/service?article_code=153645

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Original Articles

Type Code

1,268

Publication Type

Journal

Publication Title

Mansoura Veterinary Medical Journal

Publication Link

https://mvmj.journals.ekb.eg/

MainTitle

Isolation and identification of Marek’s disease virus (MDV) from feather follicle epithelium and internal organs of diseased chickens in Dakahlia Governorate, Egypt

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Article

Created At

22 Jan 2023