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185671

Conventional and Molecular Detection of Avipoxviruses from Chickens, Pigeons and Turkeys

Article

Last updated: 22 Jan 2023

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Tags

Virology

Abstract

In the present study, a total of 90 cutaneous lesions samples were collected from chickens, pigeons, and turkeys farms in Dakahlia Governorate, Egypt during summer 2016. These farms suspected to be infected with Avipoxviruses (APVs).Thirty pooled samples were created (10 from chickens, 10 from pigeons and 10 from turkeys). Hyperimmune serum was prepared against standard fowlpox virus in adult white New Zealand rabbits. APV were identified in the collected samples using agar gel precipitation test (AGPT), indirect immunoperoxidase, and polymerase chain reaction (PCR) based on 4b gene of APVs. The results revealed that out of 30 tested samples there were 16 samples (53.3%) tested positive via AGPT including, 6 chicken samples (60%) , 5 pigeon samples (50%) and 5 turkey samples (50%). while using indirect immunoperoxidase, positive results were detected in 23 samples (76.7%) including, 8 chicken samples (80%), 8 pigeon samples (80%) and 7 turkey samples (70%).The 4b gene of APVs was detected using PCR in all tested samples (100%). In conclusion, Indirect immunoperoxidase is superior over AGPT in APVs detection in collected samples from chickens, pigeons and turkeys. PCR could efficiently used in molecular diagnosis of the virus.

DOI

10.21608/mvmj.2019.01.1014

Keywords

Avipoxviruses, chickens, turkey, Egypt

Authors

First Name

Samah

Last Name

Mosad

MiddleName

-

Affiliation

Department of Virology, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt

Email

dr.sama786@yahoo.com

City

-

Orcid

https://orcid.org/00

First Name

Mohamed

Last Name

El-Tholoth

MiddleName

-

Affiliation

Department of Virology, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt

Email

-

City

-

Orcid

-

First Name

Ali

Last Name

El-Kenawy

MiddleName

-

Affiliation

Department of Virology, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt

Email

-

City

-

Orcid

-

Volume

20

Article Issue

1

Related Issue

22626

Issue Date

2019-03-01

Receive Date

2019-01-08

Publish Date

2019-03-24

Page Start

85

Page End

91

Print ISSN

1110-7219

Online ISSN

2682-2512

Article File

MVMJ_Volume 20_Issue 1_Pages 85-91.pdf

PDF . 762.7kB

Link

https://mvmj.journals.ekb.eg/article_185671.html

Detail API

https://mvmj.journals.ekb.eg/service?article_code=185671

Order

21

Type

Original Articles

Type Code

1,268

Publication Type

Journal

Publication Title

Mansoura Veterinary Medical Journal

Publication Link

https://mvmj.journals.ekb.eg/

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-

Details

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Article

Created At

22 Jan 2023