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153649

The effect of activated pure platelete rich plasma (P-PRP) on the proliferation of adipose -derived mesenchymal stem cells (AD-MSCs) as a substitute for fetal bovine serum (FBS)

Article

Last updated: 22 Jan 2023

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-

Tags

Anatomy and histology

Abstract

The field of stem cells has attracted the attention of many researchers as a hope to treat many incurable diseases because of their ability of self-renewal and differentiation into a specific type of cells that compensates damaged cells. Many studies have been published to confirm their effectiveness but there are some obstacles that limit their clinical applications. One of these obstacles is xenogenic fetal bovine serum (FBS); the main proliferative source for stem cell culture with subsequent risk of infection transmission or immunogenic problems. So, in our study, we aimed to replace the xenogenic FBS with xeno-free blood derivative. We tested the effect of different concentrations of activated pure platelet rich plasma (P-PRP); one of the blood derivatives on proliferation of adipose derived mesenchymal stem cells (AD-MSCs) in comparison to FBS and found that 20% activated P-PRP followed by 10% activated P-PRP increased the proliferation rate of AD-MSCs more than 10% FBS. KEYWORDS AD-MSCs, P-PRP, FBS.

DOI

10.21608/mvmj.2019.01.103

Keywords

AD-MSCs, P-PRP, FBS

Authors

First Name

Aya

Last Name

El-Gamal

MiddleName

-

Affiliation

Department of Cytology and Histology, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt

Email

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City

-

Orcid

-

First Name

Mohammed

Last Name

Kassab

MiddleName

-

Affiliation

Department of Cytology and Histology, Faculty of Veterinary Medicine, Kafrelshiekh University, Kafrelshiek 33511, Egypt

Email

-

City

-

Orcid

-

First Name

Ahmed

Last Name

Abd-Elmaksoud

MiddleName

-

Affiliation

Department of Cytology and Histology, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt

Email

-

City

-

Orcid

-

First Name

Amany

Last Name

Farag

MiddleName

-

Affiliation

Department of Cytology and Histology, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt

Email

-

City

-

Orcid

-

First Name

Hany

Last Name

Marie

MiddleName

-

Affiliation

Department of Cytology and Histology, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt

Email

-

City

-

Orcid

-

Volume

20

Article Issue

1

Related Issue

22626

Issue Date

2019-03-01

Receive Date

2019-01-09

Publish Date

2019-03-27

Page Start

35

Page End

40

Print ISSN

1110-7219

Online ISSN

2682-2512

Article File

MVMJ_Volume 20_Issue 1_Pages 35-40.pdf

PDF . 612.8kB

Link

https://mvmj.journals.ekb.eg/article_153649.html

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https://mvmj.journals.ekb.eg/service?article_code=153649

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Type

Original Articles

Type Code

1,268

Publication Type

Journal

Publication Title

Mansoura Veterinary Medical Journal

Publication Link

https://mvmj.journals.ekb.eg/

MainTitle

-

Details

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Article

Created At

22 Jan 2023