147 samples were collected from apparently healthy buffaloes and buffaloes suffering from various degrees of endometritis in dairy farms at Damieta and Dakahlia Governorates as well as from Mansoura abattoir. The isolated bacteria from healthy buffaloes were E. coli (20.5 %), S. aureus (15.4 %), K. oxytoca and S. epidermidis (10.3%), (7.7%) for each of , P. mirabilis, M. nishinomiyoensis and E. faecalis and (5.1%) for each of P. vulgaris, P. rettegri, C. freundii and P. aeruginosa. In endometritis cases, it was noticed that 30.2% of E. coli, (19.1%) S. aureus, (8.7%) S. pyogenes, (5.8) A. bovis, (5.2%) for each of K. oxytoca, E. agglomerans and K. pneumoniae, (4.6%) P. aeruginosa, (3.5%) for each of P. vulgaris and C. freundii, (2.3%) for each of P. mirabilis and Salmonellae, (1.8%) for each of Y. pseudotuberculosis and A . pyogenes and (1.2%) E. faecalis. . Histopathological study to sample collected from healthy cyclic buffaloes revealed normal endometrium. Microscopic lesions of acute endometritis revealed stromal oedema, congestion of blood vessels, mild to severe neutrophilic infiltration and mild perivascular and periglandular fibrosis. In chronic endometritis, extensive stromal fibrosis around blood vessels and gland, and diffuse mononuclear cell infiltration mostly lymphocytes and plasma cells. Sensitivity of the most prevalent isolates recovered from endometritis to 21 different types of antibiotics revealed that they were highly sensitive to enrofloxacin (96.38%), gentamicin (85.54%), chloramphenicol (83.13%), norofloxacin (80.72%) and pipracillin (78.31%), but, they were highly resistant to pencillin G (10.84%). Also, they were resistant to ampicillin (32.53%), streptomycin (36.14%), oxytetracycline (37.35%), SXT (39.7%) and lincospectinomycin (44.58 %). ,