In this study A total of 1531 lactating cows, 135 buffaloes and 323 ewes from Dakahlia Governorate as well as 3296 lactating cows, 180 buffaloes and 150 ewes from Damietta Governorate were employed in this study to determine the prevalence of brucellosis, 110 (7.18%) positive reactors from cattle, 15(11.11%) positive reactors from buffaloes and 14 (4.33%) positive reactors from sheep in Dakahlia Governorate , while positive reactors were 346 (10.49%), 10(5.55%) and 10 (6.66%), for cattle, buffaloes and sheep respectively in Damietta Governorate .The overall Prevalence of brucellosis among cattle, buffaloes and sheep in both Governorates were 456(9.44%), 25 (7.93%) and 24 (5.07%) respectively. Which in turn confirm that brucellosis is one of the important persistent and endemic diseases that has been reported at variable prevalence in Egypt. In this study some of the serologically positive animal cases from cattle, buffaloes and sheep were observed to detect the different symptoms of brucellosis infection in different animal species. Which ranged from abortion, retention of placenta, endometritis and infertility troubles. Clarifying the endemic situation of brucellosis in area of study.