124706

Comparative study on the use of different solvents, and their effect on the stability of ppr attenuated vaccine

Article

Last updated: 04 Jan 2025

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Tags

Surgery

Abstract

Paste des petils ruminants (PPR) virus as one of Morbillivirus (Paramyxoviridae) is a heat labile virus. In Egypt, as a hot-country-in general we are urgently in need to find out a suitable solvent that may be act as heat tolerant. In this work, we tried to select the best solvent which provide a longer stability of reconstituted PPR vaccine six new solvents were studied in a comparative with the traditional control solvent (physiological saline solution). These solvent soilitions included physiological saline solution, 1 M magnesium sulphate solution (MgSO4). 2.5% lactalbumin hydrolysate solution. a mix-ture of lactalbumin hydrolysate 2.5% and sucrose 5% solution 5% sucrose solution. A mixture of 1 M magnesium sulphate and saline solution. and dimethyl sulfoxide 2.5% solution. By using these solvents solutions. the reconstituting PPR vaccine was kept on ice and at 37C, titration of the virus infectivity on Vero cells was carried out at 0, 1 and 2 hours post reconstitution . it was revealed that the best solvent solution was the mixture of 2.5% lactalbumin hydrolysate and 5% sucrose that could keep the loss in the original virus titer at the most minimum.

DOI

10.21608/mvmj.2005.124706

Volume

7

Article Issue

1

Related Issue

18666

Issue Date

2005-06-01

Receive Date

2005-01-22

Publish Date

2005-06-01

Page Start

1

Page End

8

Print ISSN

1110-7219

Online ISSN

2682-2512

Link

https://mvmj.journals.ekb.eg/article_124706.html

Detail API

https://mvmj.journals.ekb.eg/service?article_code=124706

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1

Type

Original Articles

Type Code

1,268

Publication Type

Journal

Publication Title

Mansoura Veterinary Medical Journal

Publication Link

https://mvmj.journals.ekb.eg/

MainTitle

Comparative study on the use of different solvents, and their effect on the stability of ppr attenuated vaccine

Details

Type

Article

Created At

22 Jan 2023