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122777

BOVINE LEPTIN: EXPRESSION, CLONING AND PRODUCTION OF PURIFIED PROTEIN

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Last updated: 04 Jan 2025

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Tags

Theriogenology

Abstract

Leptin is an adipocyte-specific protein that junctions as an ad ipostat. Leptin is impli­ cated mainly in the regulation of feeding behav iour and the. hypothalamic pituitary­ adrenal axis ( HPA). Leptin correlate closely with the degree of adiposity and decrease acutely w ith caloric restri ction. In this study , we tested the bov ine leptin expression in different tissues of cows using RT-PCR analysis. Moreover, bovine leptin cloning and purification was carried out throughout this study. The obtained results indicated that bovine lepti11 was highly expressed in the adipose tissue mammary gland and not ex­ pressed in any other organs tested. Also bovine leptin was cloned into pGEM-T easy vector and expressed into expression vector then transfected into expression host cells. The protein jrom the induced cells was purified and confirmed using western blot anal­ ysis and SDS-PAGE. The results in this study suggest that bov ine leptin besides its role inther egulation of energy homeostasis play s a role in mammary gland growth and de­ velopment

DOI

10.21608/mvmj.2003.122777

Keywords

Bovine leptin, protein

Volume

5

Article Issue

1

Related Issue

17944

Issue Date

2003-06-01

Receive Date

2003-01-10

Publish Date

2003-06-01

Page Start

27

Page End

36

Print ISSN

1110-7219

Online ISSN

2682-2512

Article File

article_122777_7cf5f6d4722e5e1954c8224f1e0ef4c4.pdf

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Link

https://mvmj.journals.ekb.eg/article_122777.html

Detail API

https://mvmj.journals.ekb.eg/service?article_code=122777

Order

6

Type

Original Articles

Type Code

1,268

Publication Type

Journal

Publication Title

Mansoura Veterinary Medical Journal

Publication Link

https://mvmj.journals.ekb.eg/

MainTitle

BOVINE LEPTIN: EXPRESSION, CLONING AND PRODUCTION OF PURIFIED PROTEIN

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Type

Article

Created At

22 Jan 2023