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119360

Trials for preparation of hyperimmune serum against canine distemper virus conjugated with flourescine isothiocyanate

Article

Last updated: 04 Jan 2025

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Tags

Virology

Abstract

Hyper – immune serum conjugated with flourescein isothiocyanate (FIT) against canine distemper virus (CDV) was prepared. Canine hyperimmunoglobulin against (CDV) was prepared in susceptible puppies. Immunoglobulin was separated from the blood serum by concentrated ammonium sulphate solution, then purified and conjugated with FIT. The non – specific fluorescence was removed from the conjugate using mice liver powder. The prepared CDV immunoglobulin conjugated with FIT was tested for its specificity using different smears of CDV antigen with control non inoculated smears. Clear positive reactions were obtained. The prepared conjugated hyperimmune serum gave a positive reaction up to a dilution of 1 : 32.

DOI

10.21608/mvmj.1999.119360

Keywords

Viccines, Distemper, Distemper virus, canine

Authors

First Name

Guirguis

Last Name

W

MiddleName

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Orcid

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First Name

M

Last Name

Khodeir

MiddleName

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Affiliation

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Email

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City

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Orcid

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First Name

Y

Last Name

Hbashi

MiddleName

-

Affiliation

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Email

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City

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Orcid

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First Name

S

Last Name

El-Nakashly

MiddleName

-

Affiliation

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Email

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City

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Orcid

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Volume

1

Article Issue

1

Related Issue

17828

Issue Date

1999-06-01

Receive Date

1999-02-06

Publish Date

1999-06-01

Page Start

67

Page End

75

Print ISSN

1110-7219

Online ISSN

2682-2512

Link

https://mvmj.journals.ekb.eg/article_119360.html

Detail API

https://mvmj.journals.ekb.eg/service?article_code=119360

Order

14

Type

Original Articles

Type Code

1,268

Publication Type

Journal

Publication Title

Mansoura Veterinary Medical Journal

Publication Link

https://mvmj.journals.ekb.eg/

MainTitle

Trials for preparation of hyperimmune serum against canine distemper virus conjugated with flourescine isothiocyanate

Details

Type

Article

Created At

22 Jan 2023