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127703

MOLECULAR DETECTION AND SEROTYPING OF FOOT AND MOUTH DISEASE VIRUS IN TONGUE EPITHELIUM SAMPLES

Article

Last updated: 04 Jan 2025

OverviewSimilar Items

Subjects

-

Tags

Virology

Abstract

The aim of the present study is molecular identification and typing of FMDV circulating in Dakahlia governorate in Egypt, 2014. The study was accomplished on pooled nine tongue epithelium samples collected from diseased cattle and buffaloes expected to be infected with FMDV. The diseased animals showed fever , excessive salivation and ulceration on tongue and gum. Conventional reverse transcription polymerase chain reaction (RT-PCR) was used for identification of FMDV using universal primers. Then serotyping of the identified virus was done using RT-PCR for serotype O and A and Real time RT-PCR for serotype SAT2 . FMDV was identified in all the examined nine samples. Five of them were of serotype O, two of serotype A, one of serotype SAT2 and one sample was neither O, A nor SAT2. In conclusion, molecular techniques are sensitive and rapid methods for FMDV detection. In addition, serotypes O, A and SAT2 are circulating in Dakahlia Governorate, Egypt, 2014.

DOI

10.21608/mvmj.2017.127703

Keywords

FMD, Egypt, serotype

Authors

First Name

A.

Last Name

El-Kenawy

MiddleName

A.

Affiliation

Department of Virology, Faculty of Veterinary Medicine , Mansoura University. Phone: +20-50-2372593, Fax: +20-50-237995

Email

-

City

-

Orcid

-

First Name

E.

Last Name

Younis

MiddleName

E.

Affiliation

Department of Internal Medicine, Infectious and Fish diseases, Faculty of Veterinary Medicine, Mansoura University.

Email

-

City

-

Orcid

-

First Name

Hiam

Last Name

Fakhry

MiddleName

M.

Affiliation

Veterinary Serum and Vaccine Research Institute, FMD Department, Abbassia, Cairo, Egypt

Email

-

City

-

Orcid

-

First Name

R.

Last Name

Karam

MiddleName

-

Affiliation

Department of Virology, Faculty of Veterinary Medicine , Mansoura University. Phone: +20-50-2372593, Fax: +20-50-2379952

Email

-

City

-

Orcid

-

Volume

18

Article Issue

1

Related Issue

17809

Issue Date

2017-06-01

Receive Date

2017-09-12

Publish Date

2017-12-12

Page Start

397

Page End

404

Print ISSN

1110-7219

Online ISSN

2682-2512

Article File

MVMJ_Volume 18_Issue 1_Pages 397-404.pdf

PDF . 500.7kB

Link

https://mvmj.journals.ekb.eg/article_127703.html

Detail API

https://mvmj.journals.ekb.eg/service?article_code=127703

Order

42

Publication Type

Journal

Publication Title

Mansoura Veterinary Medical Journal

Publication Link

https://mvmj.journals.ekb.eg/

MainTitle

MOLECULAR DETECTION AND SEROTYPING OF FOOT AND MOUTH DISEASE VIRUS IN TONGUE EPITHELIUM SAMPLES

Details

Type

Article

Created At

22 Jan 2023