127676

DETECTION FOR THE MOST SUITABLE ANTIBIOTIC AND DISINFECTANT FOR PSEUDOMONAS AERUGINOSA ISOLATED from PNEUMONIC AND APPARENTLY HEALTHY FARM ANIMALS

Article

Last updated: 04 Jan 2025

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Internal Medicine and Infectious Diseases

Abstract

The present study was carried out on 550 samples (nasal, tracheal swabs and lung tissues) collected from pneumonic and apparently healthy cows and buffaloes. The bacteriological examination revealed that Pseudomonas aeruginosa was isolated from 172(41.1%) out of 419 cow's samples and from 46(35.1%) out of 131 buffalo's samples. from 172 cow's isolates there were 144 isolates related to serotype (1) and 28 isolates were untypable while buffalo's positive isolates revealed that 40 isolates were belonging to serotype (1) and 6 isolates were untypable. The effect of disinfectants as phenol, formalin and ethyl alcohol on viability of tested strains showed that 3.0% phenol could kill all tested strains after 5 minutes, but 3% concentration of formalin has a marked killing effect on the growth of P. aeruginosa within 10–15 minutes, while 40% ethyl alcohol dilution could kill P. aeruginosa after 10 minute. Forty P. aeruginosa isolates were tested for antibiotic susceptibility to 12 chemotherapeutic agents. The most effective antibiotic was gentamycin (95.0%) then streptomycin (87.5%) and amikacin (75%), all tested strains were completely resistant to tetracycline, cephalothin and ampicillin. It is concluded that P. aerouginosa is one of the most important cause of pneumonia in cows and buffaloes also it resists many disinfectants and remain viable except by using appropriate concentration. In vitro antibiotic sensitivity test showed that most strains were susceptible to gentamycin and amikacin although they resist many types of antibiotics.

DOI

10.21608/mvmj.2017.127676

Keywords

Pseudomonas aeruginosa, pneumonia, calves

Authors

First Name

Neermin.

Last Name

Ibrahim

MiddleName

A.

Affiliation

Dep. of Bacteriology, Mycology and Immunology, Fac. Vet. Med, Mans. Univ.

Email

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City

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Orcid

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First Name

Yasser

Last Name

Elnaker

MiddleName

-

Affiliation

Dep. of animal medicine, (Infectious Diseases) Fac. Vet. Med. Assiut University

Email

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City

-

Orcid

-

First Name

Salwa

Last Name

H

MiddleName

M

Affiliation

Dep. of Bacteriology, Mycology and Immunology, Fac. of Vet. Med. Kafrelsheikh Univ.

Email

-

City

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Orcid

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First Name

Ahmed.

Last Name

A.

MiddleName

M. A.

Affiliation

Dep. of Microbiology, Fac. Vet. Med, ZagazigUniv.

Email

-

City

-

Orcid

-

Volume

18

Article Issue

1

Related Issue

17809

Issue Date

2017-06-01

Receive Date

2017-09-12

Publish Date

2017-12-12

Page Start

405

Page End

417

Print ISSN

1110-7219

Online ISSN

2682-2512

Link

https://mvmj.journals.ekb.eg/article_127676.html

Detail API

https://mvmj.journals.ekb.eg/service?article_code=127676

Order

41

Publication Type

Journal

Publication Title

Mansoura Veterinary Medical Journal

Publication Link

https://mvmj.journals.ekb.eg/

MainTitle

DETECTION FOR THE MOST SUITABLE ANTIBIOTIC AND DISINFECTANT FOR PSEUDOMONAS AERUGINOSA ISOLATED from PNEUMONIC AND APPARENTLY HEALTHY FARM ANIMALS

Details

Type

Article

Created At

22 Jan 2023