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127671

PREVALENCE AND VIRULENCE PROFILE OF AVIAN PATHOGENIC E.COLI ISOLATED from CLINICALLY DISEASED BROILER CHICKEN

Article

Last updated: 22 Jan 2023

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Biochemistry and Physiology

Abstract

Escherichia coli plays a part in maintaining intestinal physiology. Although, there are pathogenic strains that cause different syndromes of diarrheal disease. E. coli strains are the cause of a number of diseases in broiler chickens, leading to death, decrease in production or condemning of carcasses. This study aimed to determine phenotypes and virulence associated genes of avian pathogenic Escherichia coli (APEC) recovered from diseased and freshly dead chickens including, shiga toxin 1, shiga toxin 2 and intimin using genotypic methods. A total of 200 chicken visceral organs (liver, lungs, spleen and heart) collected from diseased and freshly dead birds (four organs per each bird) were subjected for conventional culturing, serotyping and molecular CHARacterization of virulence genes. 32 (16%) of isolates were biochemically identified as E.coli and confirmed by PCR using phoA gene. O78, .O8, O145 and O1 are the most prevalent serotypes from APEC isolates. stx1 and stx2 were determined by multiplex PCR , while eae gene was detected by uniplex PCR . stx2 gene was detected in 50% ( 16/32), while, 81.26% (26/32) of APEC isolates harbored eae. stx1 was not detected in all the tested isolates. The obtained results demonstrated the importance of studies in E. coli of avian origin in regions associated with intensive poultry industry, aiming to evaluating the predominant strains and also acquiring preventive measures to minimize losses due to APEC

DOI

10.21608/mvmj.2017.127671

Keywords

E.coli, Broilers, Virulence

Authors

First Name

Gamal

Last Name

Younis

MiddleName

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Affiliation

Bacteriology, Mycology and Immunology Department Faculty of Veterinary Medicine, Mansoura University, Mansoura, 35516, Egypt.

Email

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Orcid

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First Name

Amal

Last Name

Awad

MiddleName

-

Affiliation

Bacteriology, Mycology and Immunology Department Faculty of Veterinary Medicine, Mansoura University, Mansoura, 35516, Egypt

Email

-

City

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Orcid

-

First Name

Samah

Last Name

Samir

MiddleName

-

Affiliation

Bacteriology, Mycology and Immunology Department Faculty of Veterinary Medicine, Mansoura University, Mansoura, 35516, Egypt

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-

City

-

Orcid

-

Volume

18

Article Issue

1

Related Issue

17809

Issue Date

2017-06-01

Receive Date

2017-09-12

Publish Date

2017-12-12

Page Start

419

Page End

430

Print ISSN

1110-7219

Online ISSN

2682-2512

Article File

MVMJ_Volume 18_Issue 1_Pages 419-430.pdf

PDF . 557.0kB

Link

https://mvmj.journals.ekb.eg/article_127671.html

Detail API

https://mvmj.journals.ekb.eg/service?article_code=127671

Order

40

Publication Type

Journal

Publication Title

Mansoura Veterinary Medical Journal

Publication Link

https://mvmj.journals.ekb.eg/

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Article

Created At

22 Jan 2023