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127392

IDENTIFICATION OF SINGLE NUCLEOTIDE POLYMORPHISMS IN EXON 2 OF BONE MORPHOGENETIC PROTEIN 15 GENE IN EGYPTIAN BUFFALOES

Article

Last updated: 22 Jan 2023

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-

Tags

Genetics

Abstract

Bone Morphogenetic Protein 15 (BMP 15) is a member of transforming growth factor-β (TGF- β) superfamily which plays an important role in ovarian follicular development and ovulation rate. In this study a partial sequence of this gene was screened for polymorphisms. PCR amplification of DNA isolated from blood of 100 Egyptian buffaloes hiefers (50 infertile and 50 normal). Revealed two loci, BMP15.1 (222 bp) and BMP15.2 (222 bp) which includes exon 2 of BMP 15 gene. Polymorphisms in these two loci were detected by using direct sequencing and single strand conformational polymorphism (SSCP) technique. A single pattern of SSCP was detected. This was confirmed by nucleotide sequencing which revealed absence of single nucleotides polymorphisms (SNPs) or any other type of polymorphisms in the two loci. The sequencing and PCR-SSCP patterns revealed no polymorphism in all studied animals. Further studies must be done on another loci on BMP 15 gene or other genes related to infertility in Egyptian buffaloes

DOI

10.21608/mvmj.2017.127392

Keywords

Buffalo, Egypt, Infertility, BMP15

Authors

First Name

Khairy

Last Name

El-Bayomi

MiddleName

M

Affiliation

Department of Animal Wealth Development, Faculty of Veterinary Medicine, Zagazig University, Egypt

Email

-

City

-

Orcid

-

First Name

Iman

Last Name

El Araby

MiddleName

E

Affiliation

Department of Animal Wealth Development, Faculty of Veterinary Medicine, Zagazig University, Egypt

Email

-

City

-

Orcid

-

First Name

Ayman

Last Name

Saleh

MiddleName

A

Affiliation

Department of Animal Wealth Development, Faculty of Veterinary Medicine, Zagazig University, Egypt

Email

-

City

-

Orcid

-

First Name

Hoda

Last Name

Osman

MiddleName

Z

Affiliation

Animal Production Research Institute (APRI), Agricultural Research Centre, Ministry of Agriculture, Egypt

Email

-

City

-

Orcid

-

First Name

Mahmoud

Last Name

Eltarabany

MiddleName

S

Affiliation

Department of Animal Wealth Development, Faculty of Veterinary Medicine, Zagazig University, Egypt

Email

-

City

-

Orcid

-

First Name

Ashraf

Last Name

Awad

MiddleName

-

Affiliation

Department of Animal Wealth Development, Faculty of Veterinary Medicine, Zagazig University, Egypt

Email

-

City

-

Orcid

-

First Name

Amir

Last Name

Abd El-fattah

MiddleName

H

Affiliation

Department of Animal Wealth Development, Faculty of Veterinary Medicine, Zagazig University, Egypt

Email

-

City

-

Orcid

-

First Name

Mohammed

Last Name

Abu El-Magd

MiddleName

-

Affiliation

Department of Anatomy & Embryology, Faculty of Veterinary Medicine, Kafrelsheikh University.

Email

-

City

-

Orcid

-

Volume

18

Article Issue

1

Related Issue

17809

Issue Date

2017-06-01

Receive Date

2017-09-12

Publish Date

2017-12-12

Page Start

235

Page End

242

Print ISSN

1110-7219

Online ISSN

2682-2512

Article File

MVMJ_Volume 18_Issue 1_Pages 235-242.pdf

PDF . 1.5MB

Link

https://mvmj.journals.ekb.eg/article_127392.html

Detail API

https://mvmj.journals.ekb.eg/service?article_code=127392

Order

27

Publication Type

Journal

Publication Title

Mansoura Veterinary Medical Journal

Publication Link

https://mvmj.journals.ekb.eg/

MainTitle

-

Details

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Article

Created At

22 Jan 2023