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126864

IMPROVEMENT OF BUFFALO EMBRYO DEVELOPMENT IN VITRO; EFFECT OF DIFFERENT CULTURE MEDIA

Article

Last updated: 04 Jan 2025

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Tags

Theriogenology

Abstract


The aim of the present study to compare 4 different types of media, SOF, TCM-119, CR1aa and KSOM, for in vitro culture of buffalo embryo. This study was under taken in the Department of Artificial insemination and embryo transfer, Animal Reproduction Research Institute (ARRI), Al-Haram, Giza .The selected cumulus oocyte complexes(COCs) with a compact cumulus cells and evenly granulated ooplasm were in vitro matured at 38.5°C in atmosphere of 5% CO2 with high humidity .After 24h of culture, oocyte were used for in vitro fertilization . Following 18h of insemination, oocyte were randomly assigned to investigate the effect of different types of media from the zygote to day 7 post-insemination . there were no significant difference in cleavage rate among 4 media However, the morula and blastocyst stage increased significantly(P<0.05) in using SOF (26.03, 17.88% respectively) and also by using CR1aa (25.37, 16.42% respectively) compared with TCM (11.86, 5.08% respectively) and KSOM (9.62, 3.85 %respectively).There is no significant difference between SOF and CR1aa.In conclusion, the current data reveled that SOF is the best media used in embryo development in buffalo. Key words: SOF. In vitro culture media. Buffalo

DOI

10.21608/mvmj.2017.126864

Keywords

Buffalo, SOF, TCM-119, CR1aa, KSOM

Authors

First Name

Amira

Last Name

Mostager

MiddleName

-

Affiliation

Theriogenology department, Faculty of Veterinary Medicine, Mansoura University, Egypt

Email

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City

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Orcid

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First Name

Abd El-Moneim

Last Name

Montaser

MiddleName

-

Affiliation

Theriogenology department, Faculty of Veterinary Medicine, Mansoura University, Egypt

Email

-

City

-

Orcid

-

First Name

Magdy

Last Name

Badr

MiddleName

Ramdan

Affiliation

Artificial Insemination and Embryo Transfer Department, Animal Reproduction Research Institute

Email

-

City

-

Orcid

-

First Name

Abd El-Raouf

Last Name

Hegab

MiddleName

Osman

Affiliation

Biology Department, Faculty of Science, Taif University, KSA

Email

-

City

-

Orcid

-

First Name

Samy

Last Name

Zaabel

MiddleName

Moawad

Affiliation

Theriogenology department, Faculty of Veterinary Medicine, Mansoura University, Egypt

Email

samy_saabel@yahoo.com

City

-

Orcid

-

Volume

18

Article Issue

1

Related Issue

17809

Issue Date

2017-06-01

Receive Date

2017-09-12

Publish Date

2017-12-12

Page Start

45

Page End

50

Print ISSN

1110-7219

Online ISSN

2682-2512

Article File

MVMJ_Volume 18_Issue 1_Pages 45-50.pdf

PDF . 468.2kB

Link

https://mvmj.journals.ekb.eg/article_126864.html

Detail API

https://mvmj.journals.ekb.eg/service?article_code=126864

Order

18

Publication Type

Journal

Publication Title

Mansoura Veterinary Medical Journal

Publication Link

https://mvmj.journals.ekb.eg/

MainTitle

IMPROVEMENT OF BUFFALO EMBRYO DEVELOPMENT IN VITRO; EFFECT OF DIFFERENT CULTURE MEDIA

Details

Type

Article

Created At

22 Jan 2023