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126853

MOLECULAR DIAGNOSIS OF CASEOUS LYMPHADENITIS IN SHEEP AT DAKAHLIA GOVERNORATE, EGYPT

Article

Last updated: 22 Jan 2023

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Subjects

-

Tags

Internal Medicine and Infectious Diseases

Abstract

The aim of the present study was to diagnose caseous lymphadenitis in sheep by PCR assay . A total of 1257 sheep from different flocks in Dakahlia governorate were clinically examined during the period of January 2014 to February 2015. sheep were classified according to the status of infection in the flocks to infected , incontact and free ones. 37 needle biopsy samples were randomly collected from 26 diseased, 7 incontact and 4 free examined sheep for PCR assay. rpoB gene was detected in 37 samples (100%) while PLD gene was in 18 samples (48.64%), from which 16 from diseased sheep and two from incontact ones, while not detected in free sheep. The PCR assay was rapid, specific and significant technique as bacterial culture in detecting corynbacterium pseudotuberculosis in needle biopsy samples from sheep < /span>

DOI

10.21608/mvmj.2017.126853

Keywords

Caseous lymphadenitis. Corynebacterium pseudotuberculosis . Sheep Polymerase chain reaction . Lymph nodes biopsy

Authors

First Name

A

Last Name

Selim

MiddleName

m

Affiliation

Internal medicine , Infectious and Fish diseases Department ,Faculty of Veterinary Medicine ,Mansoura University

Email

-

City

-

Orcid

-

First Name

S

Last Name

Atwa

MiddleName

M

Affiliation

Internal medicine , Infectious and Fish diseases Department ,Faculty of Veterinary Medicine ,Mansoura University

Email

-

City

-

Orcid

-

First Name

A

Last Name

El-Gedawy

MiddleName

A

Affiliation

Bacteriology Department ,Animal Health Research Institute ,Dokki, Giza

Email

-

City

-

Orcid

-

First Name

A

Last Name

Younis

MiddleName

E

Affiliation

Internal medicine , Infectious and Fish diseases Department ,Faculty of Veterinary Medicine ,Mansoura University

Email

-

City

-

Orcid

-

Volume

18

Article Issue

1

Related Issue

17809

Issue Date

2017-06-01

Receive Date

2017-09-12

Publish Date

2017-12-12

Page Start

67

Page End

74

Print ISSN

1110-7219

Online ISSN

2682-2512

Article File

MVMJ_Volume 18_Issue 1_Pages 67-74.pdf

PDF . 971.2kB

Link

https://mvmj.journals.ekb.eg/article_126853.html

Detail API

https://mvmj.journals.ekb.eg/service?article_code=126853

Order

16

Publication Type

Journal

Publication Title

Mansoura Veterinary Medical Journal

Publication Link

https://mvmj.journals.ekb.eg/

MainTitle

-

Details

Type

Article

Created At

22 Jan 2023