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126603

ISOLATION AND IDENTIFICATION OF INFECTIOUS BURSAL DISEASE VIRUS (IBDV) from INTERNAL ORGANS OF VACCINATED CHICKENS IN DAKAHLIA GOVERNORATE, EGYPT

Article

Last updated: 22 Jan 2023

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Virology

Abstract

In the present study, a total of 35 samples including bursa of Fabricius, thymus, spleen,kidney and liver (7samples for each organ) were collected from vaccinated chickens flocks at Dakahlia Governorate, Egypt during the period from March 2015 to May 2015. Each sample was pooled randomly from three to five birds (25 to 44 days old). Trials for isolation of the suspected virus from the collected samples were carried out via chorioallantoic membranes (CAMs) of 9 days embryonated chicken eggs (ECEs), collected from hens free from infectious bursal disease virus (IBDV). Three egg passages were carried out for each sample. Hyperimmune serum was prepared against standard IBDV. IBDV in both field and egg passaged samples (after 3rd passage) was identified by agar gel precipitation test (AGPT) and indirect fluorescence antibody test (IFAT). The results revealed that 30 samples (85%) including 7 (70%) samples from bursa of Fabricius and 6 (60%) samples from thymus, spleen and liver and 5(50%) samples from kidney, showed positive results in virus isolation. The positive results percentage by AGPT and IFAT for field samples were 88.5% (31 out of 35samples), while after 3rd passage in ECEs were 70% (22 out of 35 samples) and 88.5%(31 out of 35samples) respectively. The results indicate that the bursa of Fabricius is the best organ for IBDV isolation and AGPT and IFAT could be used for IBDV diagnosis.

DOI

10.21608/mvmj.2017.126603

Keywords

Infectious bursal disease virus(IBDV), virus isolation, agar gel precipitation test (AGPT), indirect fluorescence antibody test (IFAT)

Authors

First Name

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Last Name

El-Kenawy

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Affiliation

Department of Virology, Faculty of Veterinary Medicine Mansoura University, Mansoura, Egypt

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Orcid

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First Name

A

Last Name

Amin

MiddleName

A

Affiliation

Department of Virology, Faculty of Veterinary Medicine Mansoura University, Mansoura, Egypt

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City

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Orcid

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First Name

M

Last Name

El-Tholoth

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Affiliation

Department of Virology, Faculty of Veterinary Medicine Mansoura University, Mansoura, Egypt

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Volume

18

Article Issue

1

Related Issue

17809

Issue Date

2017-06-01

Receive Date

2017-09-12

Publish Date

2017-12-12

Page Start

95

Page End

104

Print ISSN

1110-7219

Online ISSN

2682-2512

Article File

MVMJ_Volume 18_Issue 1_Pages 95-104.pdf

PDF . 639.6kB

Link

https://mvmj.journals.ekb.eg/article_126603.html

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https://mvmj.journals.ekb.eg/service?article_code=126603

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Publication Title

Mansoura Veterinary Medical Journal

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https://mvmj.journals.ekb.eg/

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Article

Created At

22 Jan 2023