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125849

NEURAL DIFFERENTIATION OF ADIPOSE TISSUE DERIVED MESENCHYMAL STEM CELLS

Article

Last updated: 22 Jan 2023

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Subjects

-

Tags

Anatomy and histology

Abstract

The limited neurodegenerative power, makes from neural disorders the most challenging and difficult therapy among different diseases. till now, the traditional surgical and medical interventions only slow down the progression of these neurodegenerative diseases but the number of neurons are still diminish in many patients. Now, the mesenchymal stem cell (MSC) therapy has attracted the great attention of most scientists and clinicians around the world to be used as a therapeutic potential source for different neurodegenerative disorders. Stem cells have been found all over the body and can be obtained from different tissues as; adipose tissue, bone marrow and blood. MSCs CHARacterized by high proliferation and differentiation abilities, providing a source of neurons and glia for cell replacement therapy. Also, MSCs show neuroprotective effects without any genetic modification and not show teratoma. Lately, scientist directed toward differentiation of mesenchymal stem cells toward neural cells using different growth factors and chemicals. In the current study, we have tested the effect of ATRA in a dose 10µM and heparin in a dose of 50µg as neural inducers either for dopaminergic or cholinergic neurons. The results indicate that both retinoic acid and heparin are positively neural inducers. Also found that heparin mixed with retinoic acid give better results. These results were confirmed using RT-PCR for MAP2, β-tubulinІІІ, ChAT, TH neural genes markers.

DOI

10.21608/mvmj.2017.125849

Keywords

mesenchymal stem cell, adipose tissue, Neurodegeneration

Authors

First Name

A

Last Name

El-Gamal

MiddleName

M

Affiliation

Department of Cytology and Histology Faculty of Veterinary Medicine, Mansoura University, Egypt

Email

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City

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Orcid

-

First Name

A

Last Name

Abd-Elmaksaud

MiddleName

-

Affiliation

Department of Cytology and Histology Faculty of Veterinary Medicine, Mansoura University, Egypt

Email

-

City

-

Orcid

-

First Name

A

Last Name

Farag

MiddleName

-

Affiliation

Department of Cytology and Histology Faculty of Veterinary Medicine, Mansoura University, Egypt

Email

-

City

-

Orcid

-

First Name

H

Last Name

Marei

MiddleName

E

Affiliation

Department of Cytology and Histology Faculty of Veterinary Medicine, Mansoura University, Egypt

Email

-

City

-

Orcid

-

Volume

18

Article Issue

1

Related Issue

17809

Issue Date

2017-06-01

Receive Date

2017-09-12

Publish Date

2017-12-12

Page Start

183

Page End

193

Print ISSN

1110-7219

Online ISSN

2682-2512

Article File

MVMJ_Volume 18_Issue 1_Pages 183-193.pdf

PDF . 710.2kB

Link

https://mvmj.journals.ekb.eg/article_125849.html

Detail API

https://mvmj.journals.ekb.eg/service?article_code=125849

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5

Publication Type

Journal

Publication Title

Mansoura Veterinary Medical Journal

Publication Link

https://mvmj.journals.ekb.eg/

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Details

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Article

Created At

22 Jan 2023