Beta
ManageSearchChatDiscover
125683

DIFFERENTIATION OF HUMAN OLFACTORY BULB NEURAL STEM CELLS INTO OLIGODENDROCYTES

Article

Last updated: 22 Jan 2023

OverviewSimilar Items

Subjects

-

Tags

Anatomy and histology

Abstract

Human olfactory bulb neural stem cells (OBNSCs) are multipotent cells that are capable of differentiation into neurons, astrocytes, and oligodendrocytes. In the present study, we investigated the ability of human OBNSCS to differentiate into oligodendrocytes using RT-PCR and immunocytochemistry. In the presence of fetal calf serum, all-trans retinoic acid (RA), triiodothyronine (T3), and cyclic adenosine monophosphate (cAMP), OBNSCS were differentiated into oligodendrocytes precursors as evidenced by induction and repression of oligodendrocytes chondroitin sulphate proteoglycan (NG2) and nestin genes, respectively. These finding were confirmed at the protein level using fluorescent immunocytochemistry (FICC) according to standard protocol. Taken together, the present study highlighted the ability of OBNSC to differentiate into oligodendrocytes, a finding that indicates their possible use as a promising candidate for cell-based therapy for neurodegenerative and traumatic diseases associated with myelin impairments. Abbreviations: Human olfactory bulb neural stem cells (OBNSCs), retinoic acid (RA), triiodothyronine (T3), cyclic adenosine monophosphate (cAMP), pro-oligodendrocytes chondroitin sulphate proteoglycan (NG2), immunocytochemistry (FICC).

DOI

10.21608/mvmj.2017.125683

Keywords

olfactory bulb, human, OLIGODENDROCYTES

Authors

First Name

Z.

Last Name

Shouman,

MiddleName

A

Affiliation

Department of Cytology and Histology, Faculty of veterinary medicine, Mansoura University, Egypt,

Email

-

City

-

Orcid

-

First Name

A

Last Name

Abd-Elmaksoud,

MiddleName

-

Affiliation

Department of Cytology and Histology, Faculty of veterinary medicine, Mansoura University, Egypt,

Email

-

City

-

Orcid

-

First Name

S

Last Name

Lashen

MiddleName

-

Affiliation

Department of Cytology and Histology, Faculty of veterinary medicine, Mansoura University, Egypt,

Email

-

City

-

Orcid

-

First Name

Hany

Last Name

Marei

MiddleName

E.

Affiliation

Department of Cytology and Histology, Faculty of veterinary medicine, Mansoura University, Egypt, and Biomedical Research Center, Qatar University, Doha, Qatar

Email

-

City

-

Orcid

-

Volume

18

Article Issue

1

Related Issue

17809

Issue Date

2017-06-01

Receive Date

2017-08-15

Publish Date

2017-12-12

Page Start

195

Page End

207

Print ISSN

1110-7219

Online ISSN

2682-2512

Article File

MVMJ_Volume 18_Issue 1_Pages 195-207.pdf

PDF . 882.5kB

Link

https://mvmj.journals.ekb.eg/article_125683.html

Detail API

https://mvmj.journals.ekb.eg/service?article_code=125683

Order

4

Type

Original Articles

Type Code

1,268

Publication Type

Journal

Publication Title

Mansoura Veterinary Medical Journal

Publication Link

https://mvmj.journals.ekb.eg/

MainTitle

-

Details

Type

Article

Created At

22 Jan 2023