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198486

DEVELOPMENT OF A NOVEL METABOLIC GLYCO-SENSING APPROACH WITH ANTI-AGING POTENTIAL

Article

Last updated: 22 Jan 2023

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Tags

Biochemistry and Physiology

Abstract

Senescent cells, CHARacterized by irreversible cell cycle arrest, are known to accumulate during aging and their accumulation in aged tissues, in turn, accelerates aging process and promotes age-related diseases. Therefore, targeting senescent cells is an attractive strategy for anti-aging or to delay features of aging. Here, we develop a glyco-sensing approach to specifically eliminate senescent cells using rat luminal ductal epithelial (LDE) breast milk cells as a model system. Senescent LDE cells, when treated with a metabolic sugar sialic acid (SA) under nutrient deprived condition, undergo ~3-fold (p < 0.001) more apoptosis compared to LDE adult cells as demonstrated by TUNEL assay. On SA binding lectin assays, particularly with Maackia amurensis lectin I (MAL-I), senescent cells show more sialylation compared to the adult cells, possibly causing more apoptosis on these cells. This study is the first to show that SA supplementation can be protective to aging cells experiencing nutrient scarcity, while specifically killing senescent cells implying a possible role in anti-aging. Moreover, SA supplementation under nutrient deprived condition could be a strategic marker to distinguish senescent cells from adult or aging cells.

DOI

10.21608/mvmj.2018.198486

Keywords

GLYCO, SENSING APPROACH

Authors

First Name

Dina

Last Name

AlSadek

MiddleName

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Affiliation

Department of Histology and Cytology, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44511, Egypt.

Email

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City

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Orcid

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First Name

Haitham

Last Name

Badr

MiddleName

-

Affiliation

Department of Biochemistry, Faculty of Agriculture, Zagazig University, Zagazig 44511, Egypt

Email

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City

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Orcid

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First Name

Hafiz

Last Name

Ahmed

MiddleName

-

Affiliation

GlycoMantra, Inc., Baltimore MD 21227, USA

Email

-

City

-

Orcid

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Volume

19

Article Issue

1

Related Issue

17732

Issue Date

2018-06-01

Receive Date

2018-03-14

Publish Date

2018-06-01

Page Start

127

Page End

140

Print ISSN

1110-7219

Online ISSN

2682-2512

Article File

MVMJ_Volume 19_Issue 1_Pages 127-140.pdf

PDF . 2.0MB

Link

https://mvmj.journals.ekb.eg/article_198486.html

Detail API

https://mvmj.journals.ekb.eg/service?article_code=198486

Order

18

Type

Original Articles

Type Code

1,268

Publication Type

Journal

Publication Title

Mansoura Veterinary Medical Journal

Publication Link

https://mvmj.journals.ekb.eg/

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Article

Created At

22 Jan 2023