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186523

ASSOCIATION BETWEEN POLYMORPHISMS OF LHR AND INHIB β A GENES AND FERTILITY TRAITS IN EGYPTIAN BUFFALO HEIFERS

Article

Last updated: 22 Jan 2023

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Tags

Animal welfare

Abstract

The objectives of this study were to detect polymorphisms of LHR and INHIBβA genes and their association with reproductive problems (repeat breeder syndrome) among Egyptian buffalo heifers using PCR–RFLP and DNA sequencing techniques. DNA was collected from 243 (96 normal fertile and 147 repeat breeders) Egyptian buffaloes. PCR-HhaI of 303 bp from LHR gene revealed one uncut TT band confirmed by DNA sequencing showing no SNPs in all the enrolled animals. Meanwhile PCR-StyI of 288 bp from INHIBβA gene yielded two digested (150 and 138 bp) fragments for genotype CC, three fragments (288, 150, 138 bp) for genotype CT and one undigested fragment (288 bp) for TT genotype. Results indicated that out of 96 normal fertile animals, the frequencies of CC, CT and TT genotypes were 32.3%, 39.6% and 28.1% respectively. Meanwhile 15%, 38% and 47% respectively in repeat breeder buffaloes. Frequencies of C and T alleles were 52% and 48% in normal animals and 34% and 66% in repeat breeders. Statistical analysis indicated that there was a significant association between occurance of repeat breeder and INHIBβA genotypes. DNA sequencing declared C/T SNP in intron 1 of INHIBβA gene that significantly appeared with higher frequency in repeat breeder than normal fertile helfers indicating that T allele is a risk factor and can be used in MAS for early selection of repeat breeder heifers, so improving reproductivity of Egyptian buffaloes.

DOI

10.21608/mvmj.2018.186523

Keywords

Buffalo, traits, breeding, Polymorphism

Authors

First Name

Mohamed

Last Name

Fouda

MiddleName

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Affiliation

Department of Animal Husbandry and Animal Wealth Development, Faculty of Veterinary Medicine, Mansoura University

Email

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City

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Orcid

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First Name

SH

Last Name

Hemeda

MiddleName

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Affiliation

Department of Animal Husbandry and Animal Wealth Development, Faculty of Veterinary Medicine, Mansoura University.

Email

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City

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Orcid

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First Name

Kh

Last Name

El-Bayomi

MiddleName

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Affiliation

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Email

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City

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Orcid

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First Name

I

Last Name

, El-Araby

MiddleName

-

Affiliation

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Email

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City

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Orcid

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First Name

B

Last Name

Hendam

MiddleName

-

Affiliation

Department of Animal Husbandry and Animal Wealth Development, Faculty of Veterinary Medicine, Alexandria University. 2:Department of Animal Wealth Development, Faculty of Veterinary Medicine, Zagazig University.

Email

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City

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Orcid

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Volume

19

Article Issue

1

Related Issue

17732

Issue Date

2018-06-01

Receive Date

2021-02-18

Publish Date

2018-06-01

Page Start

413

Page End

425

Print ISSN

1110-7219

Online ISSN

2682-2512

Link

https://mvmj.journals.ekb.eg/article_186523.html

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https://mvmj.journals.ekb.eg/service?article_code=186523

Order

10

Type

Original Articles

Type Code

1,268

Publication Type

Journal

Publication Title

Mansoura Veterinary Medical Journal

Publication Link

https://mvmj.journals.ekb.eg/

MainTitle

-

Details

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Article

Created At

22 Jan 2023