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185427

DIFFERENTIATION OF NESTIN-POSITIVE MESENCHYMAL STEM CELLS INTO INSULIN PRODUCING CELLS

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Last updated: 04 Jan 2025

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Tags

Bacteriology

Abstract

Diabetes constitutes a worldwide epidemic affecting all ethnic groups. Stem cell therapy provides an effective way to regenerate insulin-producing cells lost during the disease. Accordingly, we isolated and compared the differentiation ability of nestin rich bone marrow mesenchymal stem cells (MSCs) into insulin producing cells (IPCs) with the nestin poor counterpart. The purity of the isolated populations was evaluated by immunofluorescence then, cells were differentiated into IPCs. At the end of differentiation, cells were evaluated by expression of pancreatic endocrine genes, immunofluorescence as well as the release of human insulin and c-peptide according to increasing glucose concentrations that showed a significant difference. Under confocal microscope the mean percentage of IPCs was 14.3% for nestin rich MSCs while nestin poor MSCs showed 2.17 %. In conclusion, nestin positive cells is a sub-population of MSCs, capable of generating IPCs in higher percentage than its negative counterpart. Key words: Nestin; Mesenchymal stem cells; differentiation; insulin producing cells.

DOI

10.21608/mvmj.2018.185427

Keywords

MSCs, diabetes, Rats

Authors

First Name

Sahar

Last Name

Rashed

MiddleName

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Affiliation

Department of Biochemistry, Faculty of Science, Mansoura University

Email

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City

-

Orcid

-

First Name

Mahmoud

Last Name

Gabr

MiddleName

-

Affiliation

-

Email

-

City

-

Orcid

-

First Name

Abdel-Aziz

Last Name

Abdel-Aziz

MiddleName

F

Affiliation

-

Email

-

City

-

Orcid

-

First Name

Mahmoud

Last Name

Zakaria

MiddleName

-

Affiliation

-

Email

-

City

-

Orcid

-

Volume

19

Article Issue

1

Related Issue

17732

Issue Date

2018-06-01

Receive Date

2018-03-16

Publish Date

2018-06-30

Page Start

87

Page End

100

Print ISSN

1110-7219

Online ISSN

2682-2512

Link

https://mvmj.journals.ekb.eg/article_185427.html

Detail API

https://mvmj.journals.ekb.eg/service?article_code=185427

Order

8

Type

Original Articles

Type Code

1,268

Publication Type

Journal

Publication Title

Mansoura Veterinary Medical Journal

Publication Link

https://mvmj.journals.ekb.eg/

MainTitle

DIFFERENTIATION OF NESTIN-POSITIVE MESENCHYMAL STEM CELLS INTO INSULIN PRODUCING CELLS

Details

Type

Article

Created At

22 Jan 2023