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264967

Antifungal Activity of Curly Parsley Essential Oil and Its Nanoemulsion Against <i>Alternaria solani</i> and <i>Rhizoctonia solani</i> Plant Pathogens

Article

Last updated: 22 Jan 2023

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Abstract

Curly Parsley (Petroselinum crispum) seeds were planted for the purpose of essential oil extraction from dried plants by hydro-distillation. GC-MS analysis of parsley essential oil (PEO) showed that Parsley-apiol (40.42%) and myristicin (34.77%) were the major components. PEO and its nanoemulsion (PEO-NE) were tested for their antifungal activities against Alternaria solani, the causal agent of tomato early blight, and Rhizoctonia solani, the causal agent of faba bean root rot. Isolated fungi were verified by ITS1and ITS4 primers. Their partial region was deposited in the GenBank (NCBI) under OL305707 and OL305708 accession numbers for A. solani and R. solani, respectively. In vitro treatments with PEO and PEO-NE showed a significant difference in the growth of both fungi under study when compared with their negative and positive controls. PEO highest effect was recorded at 3000 mg/L while PEO-NE was recorded at 1500mg/L without significant difference from the commercial fungicide (positive control). Pathogenicity test was confirmed by evaluating the antifungal activity by detached leaf assay on tomato and radical assay on faba bean for A. solani and R. solani, respectively. This study reflects the possibility of replacing harmful fungicides with natural products, such as PEO and its nanoemulsion, against A. solani and R. solani. Eco-friendly products, e.g., natural extracts, are a must now, to be used to decrease the negative impact towards general health and pollution from using commercial chemicals.

DOI

10.21608/ejp.2022.156367.1067

Keywords

<i>Alternaria solani</i>, <i>Rhizoctonia solani</i>, Essential oils, Parsley, Nanoemulsion

Authors

First Name

Fayza

Last Name

Gomaa

MiddleName

H.

Affiliation

Seed Pathology Research Department, Plant Pathology Research Institute, Agricultural Research Center, 12619, Giza, Egypt.

Email

fayzahassan2003@yahoo.com

City

Alexandria

Orcid

0000-0002-7332-0326

First Name

Sara

Last Name

Gomaa

MiddleName

E.

Affiliation

Medicinal and Aromatic Plants Research Department, Horticulture Research Institute, Agriculture Research Center, 12619, Giza, Egypt.

Email

sara_gomaa@hotmail.com

City

Alexandria

Orcid

0000-0001-5484-2789

Volume

50

Article Issue

2

Related Issue

36189

Issue Date

2022-12-01

Receive Date

2022-09-02

Publish Date

2022-10-11

Page Start

51

Page End

62

Print ISSN

1110-0230

Online ISSN

2090-2522

Link

https://ejp.journals.ekb.eg/article_264967.html

Detail API

https://ejp.journals.ekb.eg/service?article_code=264967

Order

264,967

Type

Original Article

Type Code

1,256

Publication Type

Journal

Publication Title

Egyptian Journal of Phytopathology

Publication Link

https://ejp.journals.ekb.eg/

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Article

Created At

22 Jan 2023