In this study both Trichoderma harzianum and Bacillus megaterium. were antagonistic to the growth of Fusarium chlamydosporum, Macrophomina phaseolina and Phytophthora parasitica, the causal pathogens of plum root rot, with different degrees of inhibition. T. harzianum was the most effective in this regard compared with B. megaterium in the laboratory experiment. Also, B. megaterium was the least effective, especially with M. phaseolina. The same trend was observed in greenhouse experiment. The percentage respective disease incidence was significantly decreased by tested VAM isolate compared with the control check. Also, using T. harzianum, B. megaterium (Bio-ARC) and Trichoderma album (Bio-Zeid) decreased the percentage of disease incidence. F. chlamydosporum was the most sensitive to all bioagents followed by P. parasitica and M. phaseolina. On the other hand, the tested VAM fungus showed the highest effect against all tested root rot pathogens especially with F. chlamydosporum.