Phytostimulation microorganisms (PSM_s) vis. Bacillus subtilis, Bacillus polymyxa and Trichoderma harzianum were tested for their ability to suppress and control Fusariumroot rot in tomato plants grown in sterilized soilless medium artificially infested with Fusarium oxysporum f. sp. radicis-lycopersici (FORL). Antagonistic activities under laboratory conditions were detected at various degrees of the PSM_s against F. oxysporum. T. harzianum was the most potent antagonist and gave an inhibition value of 62%. T. harzianum was also the most effective in decreasing the percentage of pre-emergence damping-off (50%) while B. polymyxa was the most effective one in decreasing the post-emergence (3%) under greenhouse conditions.  PSM_S were used as seeds soaking or seedlings inoculation of tomato plants. Pathogenicity test showed that seedlings were more susceptible to F. oxysporum than seeds. PSM_S-treated seeds showed that T. harzianum was the superior in controlling Fusarium root rot disease (93% disease control percentage) while in case of seedlings inoculation, B. subtilis gave the highest disease control value (73.3%). B. subtilis and B. polymyxa treated seeds or seedlings were the superior in improving most growth parameters tested in both cases compared to the controls. These results suggest that the increasing of disease control and improvement of tomato growth parameters by seeds or seedlings inoculation with PSM_S may contribute to protection of tomato plants against F. oxysporum root rot disease.