Bean rust, caused by Uromyces appendiculatus, is one of the most destructive diseases of dry and snap bean production worldwide. The present study aimed to investigate the efficacy of certain fungal and bacterial bio-agents in addition to some organic acids in comparison with fungicides to control bean rust under field conditions. Only fungal and bacterial bio-agents were tested for their inhibitory effect on uredospores germination in comparison with fungicides through in vitro trial. Growth parameters, total carbohydrates, chlorophyll, protein, and enzymes activity were assessed at the end of the trials for bean plants. The obtained results of in vitro trials indicated that Sumi-8 (EC 5%) was the superior in inhibiting germination of uredospores followed by Topas 100 (EC) and B. subtilis with inhibition percentages 91, 84.6 and 81% inhibition, respectively. Results of field trials exhibited that treated bean plants with Sumi-8, Topas 100 and salicylic acid showed the lowest DS % and AUDPC. Treatments of Trichoderma viride, T. asperellum and fulvic acid displayed moderate efficacy in reducing DS % and AUDPC. All tested treatments resulted in considerable increase in growth parameters, total carbohydrates, chlorophyll, and protein in bean plants over control. Fulvic, citric and salicylic acids as well as T. viride were the most effective treatments in this regard. On the other hand, the highest activities of defense related enzymes i.e., peroxidase, polyphenoloxidase and catalase were recorded in treated bean plants with organic acids.