Synthesis of bio-nanoparticles is considered one of the most important trends for providing an eco-friendly method for nano compounds used in industry and biological purposes. Bio-nanoparticles are one of the safest important techniques. During the recent decades, many fungi have become serious and less likely to be treated with harmful fungicides, which are threatening human beings. The study tested Trichoderma viride, T. hamatum, T. harzianum, and T. koningii for the synthesis of biogenic silver nanoparticles. The bio-reduction of silver nanoparticles (AgNPs) was observed spectrophotometrically. The investigated AgNPs were characterized using ultraviolet-visible spectroscopy (UV-Vis) also, the transmission electron microscopy (TEM) and scanning electron microscopy (SEM) were followed. The synthesized AgNPs by Trichoderma spp. was observed and used as antifungal against four soil-borne Fusarium spp., F. solani, F. semitectum, F. oxysporum and F. roseum. The antifungal potency of AgNPs by Trichoderma species was evaluated against the investigated four Fusarium spp. which are considered serious soil-borne fungi.