Different pre-harvest treatments of tomato fruits, i.e. Ethiphon, Bion, potasium phosphate monobasic, potasium phosphate dibasic and indole butyric acid (IBA), were evaluated for their capability to control tomato fruit rots under naturally infection and/or artificially inoculation with Alternaria alternata, Botrytis cinerea during storage for 6 weeks throughout the two successive seasons of 2010 and 2011. All pre-harvest treatments protect tomato fruits during cold storage 10oC for 6 weeks during the two seasons. Ethiphon treatment at their three concentrations showed the higher efficacy in reducing tomatoes fruits rot which inoculated under artificial infection with B. cinerea, A. alternata and naturally infection followed by Bion treatments. There was positive relationship between increasing the concentration of pre-harvest treatments and their effect on reducing of tomatoes fruits rots during cold storage periods whether under artificial inoculated or under naturally infected during the two seasons 2010 and 2011. All treatments stimulate enzymes activities (chitinase and B-1.3-gluconase). The highest enzymes activities were obtained with Ethiphon followed by Bion compared to other treatments. Data also clearly show that, all treatment performance to enhancing the quality of tomato fruits during cold storage periods by enhancing fruits colour, increase of vitamin C contents and decrease the percentage of losses in tomato fruits fresh weight whether under artificial inoculated or under naturally infection during the two seasons 2010 and 2011.