Anthracnose is a major disease of different commercial crops. Colletotrichum spp. isolates were obtained from different fruit and vegetable plants showing anthracnose symptoms. Colletotrichum spp. isolates which were isolated from guava (Psidium guajava), mango (Mangifera indica), strawberry (Fragaria ananassa), pepper (Capsicum sp.) and tomato (Solanum lycopersicum) were used to carry out this study. Based on cultural and morphological characteristics there are large differences among fungal species of Colletotrichum spp. isolates in addition to their pathogenic effects. Colletotrichum spp. differed and distinguished according to conidial morphology. Effect of temperature degrees on mycelial growth of Colletotrichum spp. isolates from mango, guava and pepper indicate that optimal growth rates were recorded at 30ºC. However, 25ºC was the optimum temperature for strawberry fungal isolates. Host range studies reveal that mango Colletotrichum spp. isolate causing severe or moderately rot for apple, peach, mango and strawberry fruits, but appeared less symptoms on tomato and lemon fruits. This study has brought to light a wide variation in the pathogenicity, morphological physiological characters of different Colletotrichum spp. isolates.