Root-knot nematodes (Meloidogyne spp.) are the main pathogens of tomato plants (Lycopersicon esculentum) worldwide. Eight isolates of Meloidogyne javanica (Mj1, Mj2, Mj3, Mj4, Mj5, Mj6, Mj7 and Mj8) were collected from five Egyptian Governorates i.e., Ismailiya, Beni-Suef, Beheira, Minya and Alexandria. Each isolate was identified by using perineal pattern and isozyme esterase assay. These isolates showed pathogenic variability when inoculated on the tomato cultivar (Super Marmand). Mj1, Mj7 and Mj8 significantly recorded high number of galls and number of juveniles per plant while, the isolates Mj3 and Mj4 had the lowest rate at the same parameters. However, the isolates Mj2, Mj5 and Mj6 were moderately pathogenic comparing with other isolates. In addition, the tomato growth parameters, significantly affected by all isolates compared to non-infected plants. Exclusively, Mj7 isolate reduced significantly the total fresh weight and shoot dry weight compared to the other isolates