Bacillus subtilis, Paenibacillus polymyxa, Pseudomonas fluorescens and Pseudomonas putida isolates were evaluated for their biocontrol activities against fodder beet Cercospora leaf spot disease under greenhouse and field conditions compared to the fungicide Topsin M-70. β-1,3-glucanase, peroxidase, polyphenoloxidase and phenylalanine ammonia lyase as well as indolacetic acid and total phenols content were determined in treated and untreated fodder beet plants. Under greenhouse conditions, the reduction in the disease severity of the treated plants with the aforementioned bioagents ranged between 58.82 - 88.24%. Under field conditions the reduction ranged between 46.67 to 80.00% and 58.33 to 83.33% in the two locations of the experiments i.e., Nubaria and Sakha, respectively. The activities of defense-related enzymes i.e., β-1,3-glucanase, peroxidase, polyphenoloxidase and phenylalanineammonia lyase were significantly increased in all treated plants with the tested bioagents. P. fluorescens resulted in the highest activity of oxidative enzymes activity. Meanwhile, the contents of indolacetic acid and total phenols were higher in treated plants than the untreated. Also crop parameters i.e., root length, root diameter, fresh and dry weight and % dry matters were significantly increased in the treatedfodder beet plants compared to the untreated control. The tested bioagents might be playing an important role in management of Cercospora leaf spot of fodder beet plants through induction of induced systemic resistance.