Trichoderma harzianum, T. viride antagonistic fungi compatible with the antagonistic Pseudomonas fluorscens, P. putida and Basillus subtilis were evaluated as biocontrol agents against Macrophomina phaseolina, the causative pathogen of sunflower charcoal rot. No adverse interaction could be recognized among the tested Trichoderma isolates and both P. fluorescens and B. subtilis, in In vitro studies. The highest antagonistic effect against M. Phaseolina growth was revealed by B. subtilis as shown by decrease in mycelia growth and reduction in sclerotia numbers. Similar effect was recorded for T. viride and P. fluorscens. In greenhouse and field experiments T. viride and P. fluorscens and B. subtilis, along with the biocide (Rhizo-N) and fungicide (Rizolex-T) decreased the disease incidence. In this respect, T. viride mixed with either B. subtilis or P. fluorscens revealed greater effect in disease control compared to the single application by any alone, especially treatment with mixture of T. Viride and B. subtilis. The effect of the later showed disease control approximately similar to that of Rizolex-T treatments, as shown by healthy survivals and seed yield over two years study.