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89726

Role of some Non-traditional Compounds in Managing Sugar Beet Powdery Mildew

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Last updated: 22 Jan 2023

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Abstract

Certain non-traditional compounds such as compost tea, humic acid and sulphated canola oil "Sulplex." were tested as foliar application to evaluate their efficacy to induce systemic resistance against Erysiphe betae, the causal agent of powdery mildew in sugar beet (Herkl cv.) versus the traditional fungicide Opera under greenhouse and field conditions at Gemmeiza Agricultural Research Station, Agricultural Research Center (A.R.C.), Egypt during two successive winter seasons (2014/2015 and 2015/2016). Under greenhouse conditions, results revealed that spraying plants with all the tested compounds reduced disease severity of powdery mildew. The fungicide Opera was the most effective treatment in reducing disease severity and increasing the efficacy followed by "Sulplex", compost tea and humic acid. Also, all treatments significantly increased total phenols and orthodihydric (OD) phenol content of the leaves compared to the control 15, 30 and 45 days after inoculation. Also, spraying plants with the tested compounds increased the activity of peroxidase (PO), polyphenoloxidase (PPO) and chitinase enzymes compared to the control, 15, 30 and 45 days after artificial inoculation. Inthe detection of chitinase activity by using SDS-glycol chitin polyacrylamide gel electrophoresis the molecular weight for all samples ranged from 28 to 30 kDa. high intensity band expressing the highest chitinase activity was found in compost-tea treatment followed by "Sulplex". Moreover, control, fungicide Opera and humic acid treatments showed low chitinase activity. Under field experiments, results indicated that the fungicide Opera was the most effective treatment for controlling powdery mildew followed by Sulplex and compost tea. Meanwhile, humic acid was the lowest effective one in this regard. All treatments increased root yield per feddan compared to the control. The fungicide Opera and Sulplex gave the highest root yield per feddan followed by compost tea then humic acid. Moreover, compost tea and Sulplex gave the highest percentages of total soluble solids (T.S.S.), sucrose percentages in beet roots and purity.

DOI

10.21608/ejp.2017.89726

Keywords

Chitinase, sucrose%, compost tea, Erysiphe betae, Humic acid, oxidative enzymes, Phenolic compounds, powdery mildew, sugar beet and sulphated canola oil "Sulplex"

Authors

First Name

Khalid

Last Name

Eid

MiddleName

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Affiliation

Plant Pathol., Dept., Fac. Agric., Moshtohor, Benha Univ.

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Orcid

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First Name

Abdel-Nasser

Last Name

EL-Sayed

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Affiliation

Maize Sugar Crops Dis. Res. Dept., Plant Pathol. Res. Inst., A.R.C. Giza, Egypt

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Volume

45

Article Issue

1

Related Issue

13451

Issue Date

2017-06-01

Receive Date

2017-04-03

Publish Date

2017-06-30

Page Start

99

Page End

115

Print ISSN

1110-0230

Online ISSN

2090-2522

Link

https://ejp.journals.ekb.eg/article_89726.html

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https://ejp.journals.ekb.eg/service?article_code=89726

Order

7

Type

Original Article

Type Code

1,256

Publication Type

Journal

Publication Title

Egyptian Journal of Phytopathology

Publication Link

https://ejp.journals.ekb.eg/

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Article

Created At

22 Jan 2023