Forty six stem rust resistance monogenic lines (Srs) were evaluated for their resistance stability to wheat stem rust at five different environmentally diverse locations i.e., Itay El-Baroud, Minia, Sakha, Sharkyia and Beni Suef, during three successive growing seasons i.e., 2015/16, 2016/17 and 2017/18 under Egyptian field conditions. According to the stem rust response of the tested monogenic lines, Sr genes were divided into three main categories. The first category included the highest effective stem rust resistance genes; Sr2, Sr24, Sr32, Sr33, Sr36, Sr38 and Sr39. The second category contained Sr genes that showed different disease reaction (susceptible and/or resistance). While, the third category included ineffective Sr genes (susceptible) under different environments in three growing seasons. Due to the highly significance interaction between genotypes and environments, stability analysis was carried out in this study. Stability parameters show clearly that the three stem rust monogenic lines; Sr14, Sr17 and Sr37 exhibited high stability to stem rust under a wide range of environmental conditions. Eventually, the current study aimed to facilitate important information that helps plant breeders to make suitable decision in the use of these genotypes (monogenic lines) as a good source of resistance in wheat breeding program for rust resistance, especially stem rust.