Rhizoctonia solani, Fusarium solani and Macrophomina phaseolina are the most devastating and economically important in many peanut growing areas in Egypt. The effect of some essential oils, bio-control agents and Vitavax/captan on peanut damping-off was evaluated in vitro and in vivo. In vitro, the obtained results indicated that all concentrations of essential oils of Thyme, Peppermint and Lemongrass significantly reduced the growth of the tested pathogenic fungi. Thyme and Lemongrass oils at 0.50% suppressed completely the mycelia growth of the tested fungi. On the other hand, all bio-control agents decreased mycelia growth of the tested fungi in vitro and Plant guard recorded the highest reduction in growth of the pathogenic fungi. Under greenhouse conditions, results indicated that all treatments significantly reduced peanut damping-off (pre- and post-emergence). Vitavax/captan followed by Lemongrass oil and Plant guard recorded the highest reduction of damping –off incidence under greenhouse conditions. Also, the obtained data, under field conditions, indicated that all treatments significantly reduced damping-off in peanut and increased plant growth and yield in both growing seasons. Vitavax/captan followed by Lemongrass and Plant guard recorded the highest reduction of damping-off incidence and the highest increase of plant growth and yield in peanut during both growing seasons.