Background: The ethical work climate is an important element affecting nurses' professional ethical practice, commitment, and work alienation. Nurses feel alienated back to the intense workload, and exposure to infection, and have a high level of stress at work. However, nurses are expected to be professionally competent in providing high-quality care ethically, it reflects the success and durability of an organization that seeks to survive and excellence. Aim: The current study aims to systematically evaluate the correlation between nurses' perception of ethical work climate and workplace alienation at Minia University Hospital. Design: A descriptive, correlational research design was utilized to achieve the aim of the study. Method: The sample included a convenient sample of 136 nurses working in the Pediatric department at Minia University Hospital in Minia Governorate, Egypt. The study used two instruments: Ethical Climate Questionnaire, and Work Alienation Scale. Results: Illustrated that, (68.4%) of study subjects were in response to a negative ethical work climate, and (31.6%) of them had a positive ethical work climate. Moreover, (53.7%) of nurses had a high level of work alienation, as well as (39.7% & 6.6%) of them had a moderate and low level of work alienation respectively. Conclusion: Negative correlation between ethical work climate and workplace alienation of study subjects. Recommendation: Establishing a suitable environment for educational and training activities also enhances the work climate and reduces work alienation.