Background: Missed nursing care associated with devastating effects for patients, nurses, organizations, and even country so we in great need to find causes to eliminate it and its consequences. Aim: to analyze the impact of nursing work environment and patient safety culture on missed nursing care at medical and surgical departments. Methods: A descriptive correlational research design used with a convenient sample of 200 nurses providing direct nursing care for patients. The data collected from medical and surgical departments at Kafrelsheikh University Hospital, Egypt. Four tools used: nurses' personal characteristics tool, misscare survey, practice environmental scale, and nurses' perception of patient safety culture scale. Results: About one third of the nurses occasionally missed ambulating patient as ordered, turning every two hours and patient teaching (29.5%, 27.5% and 25.5%) respectively. A statistically significant inverse correlation between total score of missed nursing care, and that of both work environment and patient safety culture (r = -0.229*, p=0.001*) and (r = -0.217*, p= 0.002*) respectively. Conclusions and recommendations: Nursing work environment and patient safety culture affect missed nursing care. It is vital to regularly examine the workplace to spot any elements that can lead to more missed nursing care. Additionally, it is crucial for nurses to have ongoing training.