Background: Hydatidiform mole or (vesicular mole) results from abnormal fertilization. It is the most common disorder of gestational trophoblastic diseases and can be a partial or complete mole. The aim of this study: was to assess the effect of nursing guidelines for Improving Maternity Nurse Self-Efficacy regarding Caring Women suffering from Vesicular mole. Design: A quasi-experimental design (pre and post test) study design was utilized on 120nurses who work in Ain shams maternity hospital. Tools of data collection: The data was collected using three tools1) A Structured interviewing questionnaire sheet;2) an observation checklist for studying Nurses' practices regarding Vesicular mole; 3) a Nursing competency self-efficacy scale. Results: data analysis revealed that 66.7% of studied nurses had unsatisfactory total knowledge regarding vesicular mole before intervention and become 6,7%after implementation of the intervention. Moreover, 60% of them were have not competent practices before intervention and become 12.5% after implementation of the intervention, moreover, 31,7% of them had high self-efficacy before intervention and become 85% after implementation of the intervention. Conclusion: This study concluded that the results were support the research hypothesis Implementation of nursing guidelines about vesicular mole has improved maternity nurse self-efficacy regarding caring women suffering from vesicular mole as there was an improvement in the studied nurses' knowledge practices and self-efficacy regarding caring of women with vesicular mole. Recommendation: Performing training courses regarding vesicular mole for the nurses to increase their knowledge and practices for women diagnosed with vesicular mole