Background: Nursing is recognized as a high-stress occupation. It is essential to improve job characteristics to achieve nurses 'goals as well as organizational interests to reduce burnout which consider a major problem for nurses. Aim: Determine relationships between job characteristics and nurses' burnout in intensive care units, at both selected studied hospitals Design: A descriptive comparative co-relational design were used. Setting: Medical Specialty Hospital and Gastroenterology Surgery Center which are of centers at Mansoura University Hospitals. Subjects: All nurses working at different I.C.U at medical Specialty Hospital (36) and in Gastroenterology Surgery Center (45).Tools: Two structured questionnaires were used: Job characteristics Questionnaire and Maslash Burnout Inventory (MBI) Result: Total mean score of job characteristics at Gastroenterology Surgery Center was higher than total mean score of Medical Specialty Hospital . Level of burnout were low in all items of burnout at both setting except in relation to emotional exhaustion which was high among nurses at Medical Specialty Hospital. Conclusion: There is no significant correlation between job characteristics and level of burnout among studied nurses at Medical Specialty Hospital and Gastroenterology Surgery Center. Recommendation: The current findings suggest that intervention programs should be implemented to prevent or reduce burnout and focus on improving job characteristics among nurses working in different Intensive Care Units.