Background: Premature ovarian insufficiency (POI) has a negative effect on the quality of life and
psychological well-being of women. Aim: The current study aimed to assess the effect of nursing instructional
program on genitourinary syndrome and stress level among premature ovarian insufficiency women. Methods: A
quasi-experimental design (one-group pretest-posttest) was adopted. A purposive sample of 40 women was
recruited. The study was conducted at infertility outpatient clinics at Adam International Hospital, Cairo
governorate, Egypt. Data were collected using three tools: structured interviewing questionnaire schedule,
genitourinary syndrome assessment tool, and perceived stress scale (PSS). Results: there was statistically
significant improvement in genital symptoms as 60.0%, 75.0%, 47.5% of the women reported recurrent irritation of
vulva, vaginal dryness, and pelvic pain and pressure respectively pre-program compared to 37.5%, 50.0%, 27.5%
respectively post-program. Regarding urinary symptoms, there was a statistically significant difference between
pre- and post-program in relation to urinary frequency, urgency, and recurrent urinary infection (p= 0.001,
0.008, and 0.003 respectively). Concerning sexual function, the total mean score of female sexual function index
was significantly increased from 10.1±1.46 pre-program to 15.0±2.20 post-program (p < 0.001). As well, the
difference between stress levels pre-program and post-program was highly statistically significant (p < 0.001).
Conclusion: Application of nursing instructional program was effective in improving genitourinary syndrome
and stress level among premature ovarian insufficiency women. Recommendation: Utilization of such nursing
instructional program should be enforced to reduce suffering among POI women.