Aim of the study: This study aimed to evaluate the effectiveness of wellbeing instruction on the knowledge and attitude of university students on pre-marriage screening and counseling. Methods: A quasi-experimental design was used which was conducted at one medical Nursing and one non-medical Commerce faculty at Kafrelsheikh University, Egypt. A purposive sample of one thousand female students enrolled in this study. One tool was used which was self-administered questionnaire. Results: There was a statistical significance with total knowledge and attitude. The aggregate knowledge score expanded from 23.3% to 54% among medical students, and from 21% to 41% among non-medical students and the significance was statistically high. The elevating attitude significantly increased from 54.7% to 66% among nursing students and from 49% to 57.5% among commerce students. Conclusion: It can be inferred that, even though there was a difference in the level of knowledge for all students in the two groups after wellbeing instruction, they had inadequate information about pre-marriage screening and counseling. But above one half from each group communicated a great and motivating attitude toward pre-marriage screening and counseling. Recommendations: On the glow of these findings, it is prescribed to lead wellbeing training administrations about pre-marriage screening among students of secondary schools in Kafrelsheikh Governorate to expand the general population mindfulness and impact their states of attitude towards pre-marriage screening and counseling administrations.